TY - JOUR
T1 - Anti-CXCR3 staining is useful for detecting human cutaneous and mucosal mast cells
AU - Fukiwake, Noriko
AU - Moroi, Yoichi
AU - Imafuku, Shinichi
AU - Masuda, Teiichi
AU - Kokuba, Hisashi
AU - Furue, Masutaka
AU - Urabe, Kazunori
PY - 2006/5
Y1 - 2006/5
N2 - Human synovial mast cells (MC) can be immunolabelled with antihuman CXCR3 antibody (Ab) (clone 49801). We have investigated whether cutaneous and mucosal MC are stained with anti-CXCR3 Ab in paraffin-embedded sections. Immunohistochemical staining and immunofluorescence double staining assays were performed with anti-CXCR3, anti-tryptase, and anti-chymase Ab using normal skin, psoriatic skin lesions, and normal colon. When compared with tryptase and chymase staining, 100% of the cutaneous and 98% of the mucosal MC were positive for CXCR3. Anti-CXCR3 staining is a useful marker for human cutaneous and mucosal MC in paraffin-embedded sections.
AB - Human synovial mast cells (MC) can be immunolabelled with antihuman CXCR3 antibody (Ab) (clone 49801). We have investigated whether cutaneous and mucosal MC are stained with anti-CXCR3 Ab in paraffin-embedded sections. Immunohistochemical staining and immunofluorescence double staining assays were performed with anti-CXCR3, anti-tryptase, and anti-chymase Ab using normal skin, psoriatic skin lesions, and normal colon. When compared with tryptase and chymase staining, 100% of the cutaneous and 98% of the mucosal MC were positive for CXCR3. Anti-CXCR3 staining is a useful marker for human cutaneous and mucosal MC in paraffin-embedded sections.
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U2 - 10.1111/j.1346-8138.2006.00077.x
DO - 10.1111/j.1346-8138.2006.00077.x
M3 - Article
C2 - 16700664
AN - SCOPUS:33745315084
SN - 0385-2407
VL - 33
SP - 326
EP - 330
JO - Journal of Dermatology
JF - Journal of Dermatology
IS - 5
ER -