TY - JOUR
T1 - Anti-androgen flutamide affects gonadal development and reproduction in medaka (Oryzias latipes)
AU - Kang, Ik Joon
AU - Hano, Takeshi
AU - Oshima, Yuji
AU - Yokota, Hirofumi
AU - Tsuruda, Yukinari
AU - Shimasaki, Yohei
AU - Honjo, Tsuneo
N1 - Funding Information:
This work was funded by a grant from Ministry of the Environment, Japan.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2006
Y1 - 2006
N2 - The effects of an anti-androgenic chemical on the reproduction of medaka (Oryzias latipes) were examined. Six mating pairs of medaka were exposed to flutamide (FLU) at measured concentrations of 0.101, 0.202, 0.397, 0.787, or 1.56 mg/L for 3 weeks. During the exposure period, one female died in the 1.56-mg/L treatment group, but no lesions or pathological abnormalities were observed. The fecundity and fertility of paired medaka were significantly decreased at 1.56 mg/L compared with those of the controls. Histological examination showed intersex gonads (testis-ova) in males exposed to FLU at 0.202, 0.397, and 0.787 mg/L. However, concentrations of hepatic vitellogenin (Vtg) in both sexes were not statistically different from those in the controls. These results demonstrate that FLU affected gonadal development in male medaka and reproductive capacity in paired medaka. This is the first report of testis-ova in male medaka exposed to FLU.
AB - The effects of an anti-androgenic chemical on the reproduction of medaka (Oryzias latipes) were examined. Six mating pairs of medaka were exposed to flutamide (FLU) at measured concentrations of 0.101, 0.202, 0.397, 0.787, or 1.56 mg/L for 3 weeks. During the exposure period, one female died in the 1.56-mg/L treatment group, but no lesions or pathological abnormalities were observed. The fecundity and fertility of paired medaka were significantly decreased at 1.56 mg/L compared with those of the controls. Histological examination showed intersex gonads (testis-ova) in males exposed to FLU at 0.202, 0.397, and 0.787 mg/L. However, concentrations of hepatic vitellogenin (Vtg) in both sexes were not statistically different from those in the controls. These results demonstrate that FLU affected gonadal development in male medaka and reproductive capacity in paired medaka. This is the first report of testis-ova in male medaka exposed to FLU.
UR - http://www.scopus.com/inward/record.url?scp=33745033324&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33745033324&partnerID=8YFLogxK
U2 - 10.1016/j.marenvres.2006.04.065
DO - 10.1016/j.marenvres.2006.04.065
M3 - Article
C2 - 16712919
AN - SCOPUS:33745033324
SN - 0141-1136
VL - 62
SP - S253-S257
JO - Marine Environmental Research
JF - Marine Environmental Research
IS - SUPPL. 1
ER -