TY - JOUR
T1 - Antagonistic effects of an alternative splice variant of human IL-4, IL- 4δ2, on IL-4 activities in human monocytes and B cells
AU - Arinobu, Yojiro
AU - Atamas, Sergei P.
AU - Otsuka, Takeshi
AU - Niiro, Hiroaki
AU - Yamaoka, Kunihiro
AU - Mitsuyasu, Hiromichi
AU - Niho, Yoshiyuki
AU - Hamasaki, Naotaka
AU - White, Barbara
AU - Izuhara, Kenji
N1 - Funding Information:
1 This work was supported in part by a Research Grant for Immunology, Allergy, and Organ Transplant from the Ministry of Health and Welfare of Japan, by a grant-in-aid for Scientific Research (C) from the Ministry of Education, Science, Sports, and Culture of Japan, and by an Astra Research Grant.
PY - 1999/2/1
Y1 - 1999/2/1
N2 - IL-4 is a pleiotropic cytokine which exerts its actions on various lineages of hematopoietic and nonhematopoietic cells. This cytokine is one of the central regulators of immunity in health and disease states. An alternative splice variant, in which the second of four exons is omitted, has been recently described and designated as IL-4δ2. The variant has been previously described as a potential naturally occurring antagonist of human IL-4 (hIL-4)-stimulated T cell proliferation. In this study, we investigated the effects of recombinant human (rh) IL-4δ2 on monocytes and B cells. In monocytes, rhIL-4δ2 blocked inhibitory action of hIL-4 on LPS-induced cyclooxygenase-2 expression and subsequent prostaglandin E2 secretion. In B cells, rhIL-4δ2 was an antagonist of the hIL-4-induced synthesis of IgE and expression of CD23. Our results broaden the spectrum of hIL-4-antagonistic activities of rhIL-4δ2, thus creating the background for the potential use of rhIL-4δ2 as a therapeutic anti-hIL-4 agent.
AB - IL-4 is a pleiotropic cytokine which exerts its actions on various lineages of hematopoietic and nonhematopoietic cells. This cytokine is one of the central regulators of immunity in health and disease states. An alternative splice variant, in which the second of four exons is omitted, has been recently described and designated as IL-4δ2. The variant has been previously described as a potential naturally occurring antagonist of human IL-4 (hIL-4)-stimulated T cell proliferation. In this study, we investigated the effects of recombinant human (rh) IL-4δ2 on monocytes and B cells. In monocytes, rhIL-4δ2 blocked inhibitory action of hIL-4 on LPS-induced cyclooxygenase-2 expression and subsequent prostaglandin E2 secretion. In B cells, rhIL-4δ2 was an antagonist of the hIL-4-induced synthesis of IgE and expression of CD23. Our results broaden the spectrum of hIL-4-antagonistic activities of rhIL-4δ2, thus creating the background for the potential use of rhIL-4δ2 as a therapeutic anti-hIL-4 agent.
UR - http://www.scopus.com/inward/record.url?scp=0033082784&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033082784&partnerID=8YFLogxK
U2 - 10.1006/cimm.1998.1431
DO - 10.1006/cimm.1998.1431
M3 - Article
C2 - 9973539
AN - SCOPUS:0033082784
SN - 0008-8749
VL - 191
SP - 161
EP - 167
JO - Cellular Immunology
JF - Cellular Immunology
IS - 2
ER -
