Anionic hapten-antibody reactions on various cationic bilayer membrane systems

Hirotaka Ihara; Tomoaki Arimura; Takashi Imamura; Eiko Satoh; Toyoki Kunitake; Chuichi Hirayama

doi:10.1295/polymj.24.1017

Anionic hapten-antibody reactions on various cationic bilayer membrane systems

Hirotaka Ihara, Tomoaki Arimura, Takashi Imamura, Eiko Satoh, Toyoki Kunitake, Chuichi Hirayama

Institute for Advanced Study

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Antigen-antibody reactions using N^α, N^ε-bis(2,4-dinitrophenyl) l-lysine as an anionic hapten were examined in the presence of various cationic bilayer membranes. The turbidity change of the mixture provided information on the dispersion state of bilayer membranes. The degree of the turbidity change was closely related to the concentration of the haptens and the antibodies. In addition, the agglutination was remarkably dependent on the chemical structure of matrix amphiphiles, and there were two variants of the agglutination mode when the antigen-antibody reaction was examined at 35°C: one showed turbidity decrease with the hapten-antibody reaction and the other one did increase. According to DSC studies, the difference is attributable to different physical states resulting from the phase transition of matrix membranes.

Original language	English
Pages (from-to)	1017-1024
Number of pages	8
Journal	Polymer Journal
Volume	24
Issue number	10
DOIs	https://doi.org/10.1295/polymj.24.1017
Publication status	Published - Oct 1992

All Science Journal Classification (ASJC) codes

Polymers and Plastics
Materials Chemistry

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10.1295/polymj.24.1017

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title = "Anionic hapten-antibody reactions on various cationic bilayer membrane systems",

abstract = "Antigen-antibody reactions using Nα, Nε-bis(2,4-dinitrophenyl) l-lysine as an anionic hapten were examined in the presence of various cationic bilayer membranes. The turbidity change of the mixture provided information on the dispersion state of bilayer membranes. The degree of the turbidity change was closely related to the concentration of the haptens and the antibodies. In addition, the agglutination was remarkably dependent on the chemical structure of matrix amphiphiles, and there were two variants of the agglutination mode when the antigen-antibody reaction was examined at 35°C: one showed turbidity decrease with the hapten-antibody reaction and the other one did increase. According to DSC studies, the difference is attributable to different physical states resulting from the phase transition of matrix membranes.",

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T1 - Anionic hapten-antibody reactions on various cationic bilayer membrane systems

AU - Ihara, Hirotaka

AU - Arimura, Tomoaki

AU - Imamura, Takashi

AU - Satoh, Eiko

AU - Kunitake, Toyoki

AU - Hirayama, Chuichi

PY - 1992/10

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AB - Antigen-antibody reactions using Nα, Nε-bis(2,4-dinitrophenyl) l-lysine as an anionic hapten were examined in the presence of various cationic bilayer membranes. The turbidity change of the mixture provided information on the dispersion state of bilayer membranes. The degree of the turbidity change was closely related to the concentration of the haptens and the antibodies. In addition, the agglutination was remarkably dependent on the chemical structure of matrix amphiphiles, and there were two variants of the agglutination mode when the antigen-antibody reaction was examined at 35°C: one showed turbidity decrease with the hapten-antibody reaction and the other one did increase. According to DSC studies, the difference is attributable to different physical states resulting from the phase transition of matrix membranes.

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Anionic hapten-antibody reactions on various cationic bilayer membrane systems

Abstract

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