Animated crystallography of genetic code translation.

Kozo Tomita, Tomoyuki Numata, Tsutomu Fukai, Shuya Nureki, Ryuichiro Ishitani, Osamu Nureki

Research output: Contribution to journalArticlepeer-review


Accurate genetic code translation is achieved by accumulation of hyper-specific chemical reactions. Transfer RNA (tRNA) acts as an adaptor molecule to link the genetic code (in messenger RNA) to a specific amino acid. tRNA itself has no ability to specifically bind with the cognate amino acid, while aminoacyltRNA synthetases (aaRSs) strictly recognize their cognate tRNA and amino acid, to synthesize a correctlypaired aminoacyl-tRNA, a substrate for ribosome. tRNA is initially transcribed by RNA polymerase as a precursor RNA with long extensions at the 5' and 3' terminus. Maturation of tRNA to a functional RNA requires processing of the extensional sequences and post-transcriptional chemical modifications, which ensures the specific aminoacylation of tRNA by the cognate aaRS. We have solved the crystal structure of the tRNA-maturating enzymes in a complex with tRNA (precursor) to especially elucidate the sophisticated mechanism of their highly specific chemical reactions. Our goal is to provide the animated crystallography or "movie" of the dynamic processes in the genetic code translation by capturing snapshots of the chemical reaction steps by X-ray crystallography.

Original languageEnglish
Pages (from-to)101-102
Number of pages2
JournalNucleic acids symposium series (2004)
Issue number51
Publication statusPublished - 2007
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General Medicine


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