TY - JOUR
T1 - Analysis of protein interactions with two-hybrid system in cultured insect cells
AU - Mon, Hiroaki
AU - Sugahara, Ryohei
AU - Hong, Sun Mee
AU - Lee, Jae Man
AU - Kamachi, Yusuke
AU - Kawaguchi, Yutaka
AU - Kusakabe, Takahiro
N1 - Funding Information:
This work was supported in part by the Program for Promotion of Basic Research Activities for Innovative Biosciences (PROBRAIN) and by a grant from the Ministry of Agriculture, Forestry, and Fisheries of Japan (integrated research project for plant, insect, and animal using genome technology, INSECT-1201).
PY - 2009/9/15
Y1 - 2009/9/15
N2 - Many biological processes are usually coupled to the formation of protein complexes. The yeast two-hybrid system is a powerful tool for analyzing protein-protein interactions. Different patterns of protein modifications, such as glycosylation, phosphorylation, and acetylation, may affect the ability of proteins to interact. In this study, we developed the two-hybrid system that can be used in insect cells. To validate the insect two-hybrid (I2H) system, we analyzed and confirmed the known oligomer or dimer formation of silkworm Rad51 or RPA2-RPA3, respectively. The results established the feasibility of the I2H system for efficient analysis of protein interaction under conditions that closely reflect the normal physiological environment.
AB - Many biological processes are usually coupled to the formation of protein complexes. The yeast two-hybrid system is a powerful tool for analyzing protein-protein interactions. Different patterns of protein modifications, such as glycosylation, phosphorylation, and acetylation, may affect the ability of proteins to interact. In this study, we developed the two-hybrid system that can be used in insect cells. To validate the insect two-hybrid (I2H) system, we analyzed and confirmed the known oligomer or dimer formation of silkworm Rad51 or RPA2-RPA3, respectively. The results established the feasibility of the I2H system for efficient analysis of protein interaction under conditions that closely reflect the normal physiological environment.
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U2 - 10.1016/j.ab.2009.05.033
DO - 10.1016/j.ab.2009.05.033
M3 - Article
C2 - 19481053
AN - SCOPUS:67650479291
SN - 0003-2697
VL - 392
SP - 180
EP - 182
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -