Analysis of essential pathways for self-renewal in common marmoset embryonic stem cells

Takenobu Nii; Tomotoshi Marumoto; Hirotaka Kawano; Saori Yamaguchi; Jiyuan Liao; Michiyo Okada; Erika Sasaki; Yoshie Miura; Kenzaburo Tani

doi:10.1016/j.fob.2014.02.007

Analysis of essential pathways for self-renewal in common marmoset embryonic stem cells

Takenobu Nii, Tomotoshi Marumoto, Hirotaka Kawano, Saori Yamaguchi, Jiyuan Liao, Michiyo Okada, Erika Sasaki, Yoshie Miura, Kenzaburo Tani

Bioregulation

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Common marmoset (CM) is widely recognized as a useful non-human primate for disease modeling and preclinical studies. Thus, embryonic stem cells (ESCs) derived from CM have potential as an appropriate cell source to test human regenerative medicine using human ESCs. CM ESCs have been established by us and other groups, and can be cultured in vitro. However, the growth factors and downstream pathways for self-renewal of CM ESCs are largely unknown. In this study, we found that basic fibroblast growth factor (bFGF) rather than leukemia inhibitory factor (LIF) promoted CM ESC self-renewal via the activation of phosphatidylinositol-3-kinase (PI3K)-protein kinase B (AKT) pathway on mouse embryonic fibroblast (MEF) feeders. Moreover, bFGF and transforming growth factor β (TGFβ) signaling pathways cooperatively maintained the undifferentiated state of CM ESCs under feeder-free condition. Our findings may improve the culture techniques of CM ESCs and facilitate their use as a preclinical experimental resource for human regenerative medicine.

Original language	English
Pages (from-to)	213-219
Number of pages	7
Journal	FEBS Open Bio
Volume	4
DOIs	https://doi.org/10.1016/j.fob.2014.02.007
Publication status	Published - Feb 2014

All Science Journal Classification (ASJC) codes

Biochemistry, Genetics and Molecular Biology(all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.fob.2014.02.007

Cite this

@article{e0d8e961db794b87affaf0191eb00aa9,

title = "Analysis of essential pathways for self-renewal in common marmoset embryonic stem cells",

abstract = "Common marmoset (CM) is widely recognized as a useful non-human primate for disease modeling and preclinical studies. Thus, embryonic stem cells (ESCs) derived from CM have potential as an appropriate cell source to test human regenerative medicine using human ESCs. CM ESCs have been established by us and other groups, and can be cultured in vitro. However, the growth factors and downstream pathways for self-renewal of CM ESCs are largely unknown. In this study, we found that basic fibroblast growth factor (bFGF) rather than leukemia inhibitory factor (LIF) promoted CM ESC self-renewal via the activation of phosphatidylinositol-3-kinase (PI3K)-protein kinase B (AKT) pathway on mouse embryonic fibroblast (MEF) feeders. Moreover, bFGF and transforming growth factor β (TGFβ) signaling pathways cooperatively maintained the undifferentiated state of CM ESCs under feeder-free condition. Our findings may improve the culture techniques of CM ESCs and facilitate their use as a preclinical experimental resource for human regenerative medicine.",

author = "Takenobu Nii and Tomotoshi Marumoto and Hirotaka Kawano and Saori Yamaguchi and Jiyuan Liao and Michiyo Okada and Erika Sasaki and Yoshie Miura and Kenzaburo Tani",

note = "Funding Information: We thank Michiko Ushijima for administrative assistance, Yoko Nagai and the members of Tani laboratory for their constructive criticisms and technical support. This work was supported by a grant from the Project for Realization of Regenerative Medicine (K. Tani, 08008010 ) and KAKENHI (T. Marumoto, 23590465 ) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) , Japan. ",

year = "2014",

month = feb,

doi = "10.1016/j.fob.2014.02.007",

language = "English",

volume = "4",

pages = "213--219",

journal = "FEBS Open Bio",

issn = "2211-5463",

publisher = "Elsevier BV",

}

TY - JOUR

T1 - Analysis of essential pathways for self-renewal in common marmoset embryonic stem cells

AU - Nii, Takenobu

AU - Marumoto, Tomotoshi

AU - Kawano, Hirotaka

AU - Yamaguchi, Saori

AU - Liao, Jiyuan

AU - Okada, Michiyo

AU - Sasaki, Erika

AU - Miura, Yoshie

AU - Tani, Kenzaburo

N1 - Funding Information: We thank Michiko Ushijima for administrative assistance, Yoko Nagai and the members of Tani laboratory for their constructive criticisms and technical support. This work was supported by a grant from the Project for Realization of Regenerative Medicine (K. Tani, 08008010 ) and KAKENHI (T. Marumoto, 23590465 ) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) , Japan.

PY - 2014/2

Y1 - 2014/2

N2 - Common marmoset (CM) is widely recognized as a useful non-human primate for disease modeling and preclinical studies. Thus, embryonic stem cells (ESCs) derived from CM have potential as an appropriate cell source to test human regenerative medicine using human ESCs. CM ESCs have been established by us and other groups, and can be cultured in vitro. However, the growth factors and downstream pathways for self-renewal of CM ESCs are largely unknown. In this study, we found that basic fibroblast growth factor (bFGF) rather than leukemia inhibitory factor (LIF) promoted CM ESC self-renewal via the activation of phosphatidylinositol-3-kinase (PI3K)-protein kinase B (AKT) pathway on mouse embryonic fibroblast (MEF) feeders. Moreover, bFGF and transforming growth factor β (TGFβ) signaling pathways cooperatively maintained the undifferentiated state of CM ESCs under feeder-free condition. Our findings may improve the culture techniques of CM ESCs and facilitate their use as a preclinical experimental resource for human regenerative medicine.

AB - Common marmoset (CM) is widely recognized as a useful non-human primate for disease modeling and preclinical studies. Thus, embryonic stem cells (ESCs) derived from CM have potential as an appropriate cell source to test human regenerative medicine using human ESCs. CM ESCs have been established by us and other groups, and can be cultured in vitro. However, the growth factors and downstream pathways for self-renewal of CM ESCs are largely unknown. In this study, we found that basic fibroblast growth factor (bFGF) rather than leukemia inhibitory factor (LIF) promoted CM ESC self-renewal via the activation of phosphatidylinositol-3-kinase (PI3K)-protein kinase B (AKT) pathway on mouse embryonic fibroblast (MEF) feeders. Moreover, bFGF and transforming growth factor β (TGFβ) signaling pathways cooperatively maintained the undifferentiated state of CM ESCs under feeder-free condition. Our findings may improve the culture techniques of CM ESCs and facilitate their use as a preclinical experimental resource for human regenerative medicine.

UR - http://www.scopus.com/inward/record.url?scp=84896045260&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84896045260&partnerID=8YFLogxK

U2 - 10.1016/j.fob.2014.02.007

DO - 10.1016/j.fob.2014.02.007

M3 - Article

AN - SCOPUS:84896045260

SN - 2211-5463

VL - 4

SP - 213

EP - 219

JO - FEBS Open Bio

JF - FEBS Open Bio

ER -

Analysis of essential pathways for self-renewal in common marmoset embryonic stem cells

Abstract

All Science Journal Classification (ASJC) codes

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this