TY - JOUR
T1 - Analysis of cell viability and differential activity of mouse hepatocytes under 3D and 2D culture in agarose gel
AU - Ise, Hirohiko
AU - Takashima, Seiji
AU - Nagaoka, Masato
AU - Ferdous, Anwarul
AU - Akaike, Toshihiro
N1 - Funding Information:
This work was supported in part by a grant-in-aid for scientific research from the Ministry of Education, Science, Sports, and Culture of Japan.
PY - 1999
Y1 - 1999
N2 - Three-dimensional (3D) and two-dimensional (2D) cultures of hepatocytes in various concentrations (0.3-0.7%) of agarose gel revealed that the hepatocytes under 3D cultures in 0.3% agarose gel possess long-term (> 3 weeks) viability, significant self-assembly to form tissue like aggregates, low lactate dehydrogenase release and high albumin synthesis. These were in contrast to 2D culture of hepatocytes. Our results suggest that the 3D culture of hepatocytes in agarose gel favors aggregate formation of functionally active cells and would be useful for liver transplantation as well as to analyze hepatocytes biology.
AB - Three-dimensional (3D) and two-dimensional (2D) cultures of hepatocytes in various concentrations (0.3-0.7%) of agarose gel revealed that the hepatocytes under 3D cultures in 0.3% agarose gel possess long-term (> 3 weeks) viability, significant self-assembly to form tissue like aggregates, low lactate dehydrogenase release and high albumin synthesis. These were in contrast to 2D culture of hepatocytes. Our results suggest that the 3D culture of hepatocytes in agarose gel favors aggregate formation of functionally active cells and would be useful for liver transplantation as well as to analyze hepatocytes biology.
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U2 - 10.1023/A:1005412129500
DO - 10.1023/A:1005412129500
M3 - Article
AN - SCOPUS:0032929251
SN - 0141-5492
VL - 21
SP - 209
EP - 213
JO - Biotechnology letters
JF - Biotechnology letters
IS - 3
ER -