TY - CHAP
T1 - An in vitro three-dimensional organotypic model to analyze peripancreatic fat invasion in pancreatic cancer
T2 - A culture system based on collagen gel embedding
AU - Okumura, Takashi
AU - Ohuchida, Kenoki
AU - Nakamura, Masafumi
N1 - Funding Information:
We thank James P. Mahaffey, PhD, from Edanz Group (www. edanzediting.com/ac) for editing a draft of this manuscript. Grant support: This work was supported in part by Japan Society of Promoting of the Science (JSPS) Grant-in-Aid for Scientific Research (B) (Grant Number: 17H04284,16H05418).
Publisher Copyright:
© 2019, Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2019
Y1 - 2019
N2 - Three-dimensional culture systems reflect biological environments better than conventional two-dimensional culture. Additionally, three-dimensional culture is a strong experimental tool to analyze direct interactions between cancer cells and stromal cells in vitro. Herein, we describe protocols for an organotypic fat invasion model that is a novel culturing system mimicking the extrapancreatic invasion of pancreatic adenocarcinoma (PDAC). This novel model is based on the collagen I gel embedding method and enables us to analyze the functional and histological interactions between cancer cells and adipose tissue.
AB - Three-dimensional culture systems reflect biological environments better than conventional two-dimensional culture. Additionally, three-dimensional culture is a strong experimental tool to analyze direct interactions between cancer cells and stromal cells in vitro. Herein, we describe protocols for an organotypic fat invasion model that is a novel culturing system mimicking the extrapancreatic invasion of pancreatic adenocarcinoma (PDAC). This novel model is based on the collagen I gel embedding method and enables us to analyze the functional and histological interactions between cancer cells and adipose tissue.
UR - http://www.scopus.com/inward/record.url?scp=85055645712&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85055645712&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-8879-2_11
DO - 10.1007/978-1-4939-8879-2_11
M3 - Chapter
C2 - 30378049
AN - SCOPUS:85055645712
T3 - Methods in Molecular Biology
SP - 135
EP - 141
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -
