An immunochromatographic assay for rapid etection of salvinorin A

Madan Kumar Paudel; Osamu Shirota; Seiichi Sakamoto; Satoshi Morimoto; Hiroyuki Tanaka

doi:10.1080/15321819.2017.1324797

An immunochromatographic assay for rapid etection of salvinorin A

Madan Kumar Paudel, Osamu Shirota, Seiichi Sakamoto, Satoshi Morimoto, Hiroyuki Tanaka

Pharmaceutical Health Care and Sciences

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

We developed an immunochromatographic assay (ICA) that enables rapid analysis of salvinorin A (Sal A) in Salvia divinorum within 10 min. The result shows that no Sal A in other samples of Lamiaceae plants was detected, but it could recognize Sal A among other substances in complex samples. The main advantage of the ICA is its high performance in combination with low cost, simplicity, and speed. Our newly developed combined ICA/indirect competitive ELISA(icELISA) system enables analysis of large numbers of samples over short periods of time without cumbersome pretreatments in complex mixtures. This method can complement other instrumental analyses for salvinorins, and could be used to deter S. divinorum abuse.

Original language	English
Pages (from-to)	438-448
Number of pages	11
Journal	Journal of Immunoassay and Immunochemistry
Volume	38
Issue number	4
DOIs	https://doi.org/10.1080/15321819.2017.1324797
Publication status	Published - Jul 4 2017

All Science Journal Classification (ASJC) codes

Immunology and Allergy
Immunology
Clinical Biochemistry
Medical Laboratory Technology

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10.1080/15321819.2017.1324797

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title = "An immunochromatographic assay for rapid etection of salvinorin A",

abstract = "We developed an immunochromatographic assay (ICA) that enables rapid analysis of salvinorin A (Sal A) in Salvia divinorum within 10 min. The result shows that no Sal A in other samples of Lamiaceae plants was detected, but it could recognize Sal A among other substances in complex samples. The main advantage of the ICA is its high performance in combination with low cost, simplicity, and speed. Our newly developed combined ICA/indirect competitive ELISA(icELISA) system enables analysis of large numbers of samples over short periods of time without cumbersome pretreatments in complex mixtures. This method can complement other instrumental analyses for salvinorins, and could be used to deter S. divinorum abuse.",

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T1 - An immunochromatographic assay for rapid etection of salvinorin A

AU - Paudel, Madan Kumar

AU - Shirota, Osamu

AU - Sakamoto, Seiichi

AU - Morimoto, Satoshi

AU - Tanaka, Hiroyuki

PY - 2017/7/4

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AB - We developed an immunochromatographic assay (ICA) that enables rapid analysis of salvinorin A (Sal A) in Salvia divinorum within 10 min. The result shows that no Sal A in other samples of Lamiaceae plants was detected, but it could recognize Sal A among other substances in complex samples. The main advantage of the ICA is its high performance in combination with low cost, simplicity, and speed. Our newly developed combined ICA/indirect competitive ELISA(icELISA) system enables analysis of large numbers of samples over short periods of time without cumbersome pretreatments in complex mixtures. This method can complement other instrumental analyses for salvinorins, and could be used to deter S. divinorum abuse.

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An immunochromatographic assay for rapid etection of salvinorin A

Abstract

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