An expanded palette of genetically encoded Ca2+ indicators

Yongxin Zhao, Satoko Araki, Jiahui Wu, Takayuki Teramoto, Yu Fen Chang, Masahiro Nakano, Ahmed S. Abdelfattah, Manabi Fujiwara, Takeshi Ishihara, Takeharu Nagai, Robert E. Campbell

Research output: Contribution to journalArticlepeer-review

1022 Citations (Scopus)


Engineered fluorescent protein (FP) chimeras that modulate their fluorescence in response to changes in calcium ion (Ca2+) concentration are powerful tools for visualizing intracellular signaling activity. However, despite a decade of availability, the palette of single FP-based Ca2+ indicators has remained limited to a single green hue. We have expanded this palette by developing blue, improved green, and red intensiometric indicators, as well as an emission ratiometric indicator with an 11,000% ratio change. This series enables improved single-color Ca2+ imaging in neurons and transgenic Caenorhabditis elegans. In HeLa cells, Ca 2+ was imaged in three subcellular compartments, and, in conjunction with a cyan FP-yellow FP-based indicator, Ca2+ and adenosine 5′-triphosphate were simultaneously imaged. This palette of indicators paints the way to a colorful new era of Ca2+ imaging.

Original languageEnglish
Pages (from-to)1888-1891
Number of pages4
Issue number6051
Publication statusPublished - Sept 30 2011

All Science Journal Classification (ASJC) codes

  • General


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