TY - JOUR
T1 - An estimated quantitative lateral flow immunoassay for determination of artesunate using monoclonal antibody
AU - Kitisripanya, Tharita
AU - Sermpradit, Witcha
AU - Sakamoto, Seiichi
AU - Tanaka, Hiroyuki
AU - Putalun, Waraporn
N1 - Funding Information:
This work was supported by Khon Kaen University and The Thailand Research Fund (IRN61W0005), Thailand.
Funding Information:
This work was supported by Khon Kaen University and The Thailand Research Fund (IRN61W0005), Thailand.
Publisher Copyright:
© 2019 John Wiley & Sons, Ltd.
PY - 2020/2/1
Y1 - 2020/2/1
N2 - There have been reports of fake artesunate (ART), which has led to deaths from untreated malaria in South East Asia. To rapidly screen for fake and adulterated ART products in the drug market, a lateral flow immunoassay (LFIA) based on a colloidal gold–monoclonal antibody probe for detection of ART within samples was developed. With this method, the calibration curve for ART was determined by the intensity ratio of the test and control bands at various ART concentrations. The linearity range was 12.5–200 μg/ml of ART. Samples were tested by the developed LFIA and can be calculated for ART contents. The levels of ART in the samples were also confirmed by enzyme-linked immunosorbent assay. The results of the two methods were in good conformance. The proposed LFIA was demonstrated to be a simple and rapid analytical method for detecting ART in the pharmaceutical formulation.
AB - There have been reports of fake artesunate (ART), which has led to deaths from untreated malaria in South East Asia. To rapidly screen for fake and adulterated ART products in the drug market, a lateral flow immunoassay (LFIA) based on a colloidal gold–monoclonal antibody probe for detection of ART within samples was developed. With this method, the calibration curve for ART was determined by the intensity ratio of the test and control bands at various ART concentrations. The linearity range was 12.5–200 μg/ml of ART. Samples were tested by the developed LFIA and can be calculated for ART contents. The levels of ART in the samples were also confirmed by enzyme-linked immunosorbent assay. The results of the two methods were in good conformance. The proposed LFIA was demonstrated to be a simple and rapid analytical method for detecting ART in the pharmaceutical formulation.
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U2 - 10.1002/bmc.4718
DO - 10.1002/bmc.4718
M3 - Article
C2 - 31642080
AN - SCOPUS:85076762583
SN - 0269-3879
VL - 34
JO - Biomedical Chromatography
JF - Biomedical Chromatography
IS - 2
M1 - e4718
ER -
