TY - JOUR
T1 - An endocrine disruptor, bisphenol A, affects development in the protochordate Ciona intestinalis
T2 - Hatching rates and swimming behavior alter in a dose-dependent manner
AU - Matsushima, Ayami
AU - Ryan, Kerrianne
AU - Shimohigashi, Yasuyuki
AU - Meinertzhagen, Ian A.
N1 - Funding Information:
The authors wish to thank Dr. D. Sephton and Ms. J. Keays, Bedford Institute of Oceanography, Nova Scotia, for providing animals. The authors also thank Dr. Y. Satou, Kyoto University and the National BioResource Project Ciona intestinalis, for giving technical advice and providing animals. This research was supported by FY 2009 Researcher Exchange Program between the Japan Society for the Promotion of Science (JSPS) and the Natural Sciences and Engineering Research Council (NSERC) of Canada . This work was also supported in part by grants-in-aid from the Ministry of Education, Science, Sports and Culture in Japan to A.M. ( 20710053 ) and Y.S. ( 22221005 ) and by Discovery Grant 0000065 from NSERC (to IAM).
PY - 2013/2
Y1 - 2013/2
N2 - Bisphenol A (BPA) is widely used industrially to produce polycarbonate plastics and epoxy resins. Numerous studies document the harmful effects caused by low-dose BPA exposure especially on nervous systems and behavior in experimental animals such as mice and rats. Here, we exposed embryos of a model chordate, Ciona intestinalis, to seawater containing BPA to evaluate adverse effects on embryonic development and on the swimming behavior of subsequent larvae. Ciona is ideal because its larva develops rapidly and has few cells. The rate of larval hatching decreased in a dose-dependent manner with exposures to BPA above 3 μM; swimming behavior was also affected in larvae emerging from embryos exposed to 1 μM BPA. Adverse effects were most severe on fertilized eggs exposed to BPA within 7 h post-fertilization. Ciona shares twelve nuclear receptors with mammals, and BPA is proposed to disturb the physiological functions of one or more of these.
AB - Bisphenol A (BPA) is widely used industrially to produce polycarbonate plastics and epoxy resins. Numerous studies document the harmful effects caused by low-dose BPA exposure especially on nervous systems and behavior in experimental animals such as mice and rats. Here, we exposed embryos of a model chordate, Ciona intestinalis, to seawater containing BPA to evaluate adverse effects on embryonic development and on the swimming behavior of subsequent larvae. Ciona is ideal because its larva develops rapidly and has few cells. The rate of larval hatching decreased in a dose-dependent manner with exposures to BPA above 3 μM; swimming behavior was also affected in larvae emerging from embryos exposed to 1 μM BPA. Adverse effects were most severe on fertilized eggs exposed to BPA within 7 h post-fertilization. Ciona shares twelve nuclear receptors with mammals, and BPA is proposed to disturb the physiological functions of one or more of these.
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U2 - 10.1016/j.envpol.2012.10.015
DO - 10.1016/j.envpol.2012.10.015
M3 - Article
C2 - 23207495
AN - SCOPUS:84870169429
SN - 0269-7491
VL - 173
SP - 257
EP - 263
JO - Environmental Pollution
JF - Environmental Pollution
ER -