Amyloid-β detection with saccharide immobilized gold nanoparticle on carbon electrode

Miyuki Chikae, Tomohiro Fukuda, Kagan Kerman, Koutarou Idegami, Yoshiko Miura, Eiichi Tamiya

Research output: Contribution to journalArticlepeer-review

124 Citations (Scopus)


The electrochemical sensing of saccharide-protein interactions using a couple of sialic acid derivatives and Alzheimer's amyloid-beta (Aβ) is described. The densely-packed saccharide area for recognition of protein was fabricated onto a carbon electrode by three steps, which were electrochemical deposition of Au nanoparticles on a screen printed strip, self-assembled monolayer (SAM) formation of the acetylenyl group on Au nanoparticles, and the cycloaddition reaction of an azide-terminated sialic acid to the acetylenyl group. The attachment of Aβ peptides to the sialic acid layer was confirmed by electrochemistry and atomic force microscopy imaging. The intrinsic oxidation signal of the captured Aβ(1-40) and (1-42) peptides, containing a single tyrosine (Tyr) residues, was monitored at a peak potential of 0.6 V (vs Ag/AgCl within this sensor) in connection with differential pulse voltammetry. The peak current intensities were concentration dependent. The proposed process provides new routes for analysis of saccharide-protein interactions and electrochemical biosensor development.

Original languageEnglish
Pages (from-to)118-123
Number of pages6
Issue number1
Publication statusPublished - Nov 2008
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Physical and Theoretical Chemistry
  • Electrochemistry


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