TY - JOUR
T1 - Amphiregulin regulates the production of human chorionic gonadotropin in trophoblasts
AU - Fukami, Tatsuya
AU - Yoshizato, Toshiyuki
AU - Miyamoto, Shingo
AU - Yagi, Hiroshi
AU - Yotsumoto, Fusanori
AU - Nabeshima, Kazuki
AU - Hachisuga, Toru
AU - Kuroki, Masahide
AU - Kawarabayashi, Tatsuhiko
N1 - Funding Information:
This study was supported in part by funds from the “FUKUOKA” OBGYN Researcher's Charity Foundation Fund (to Yoshizato) and the Central Research Institute of Fukuoka University (to Miyamoto) and Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (to Miyamoto). We thank Dr. Eisuke Mekada, Dr. Hidetaka Izumi, Dr. Hidehito Mohtai and Dr. Masafumi Shindoh for critical comments, and Yumiko Hirose and Kyoko Yano for assistance with the sample preparation.
PY - 2009/6/5
Y1 - 2009/6/5
N2 - Aims: The aim of this study was to investigate the significance of epidermal growth factor receptor (EGFR) ligands produced in syncytiotrophoblasts during normal pregnancy. Main methods: We examined the expression of EGFR ligands in human pregnancy by real-time PCR, and analyzed the relationship between EGFR ligands and human chorionic gonadotropin (hCG) or human placental lactogen in amniotic fluid by ELISA. In addition, we also examined the EGFR ligands in syncytiotrophoblasts and the amount of hCG secretion in JAR, JEG3 and BeWo cells in the presence of each EGFR ligand. Key findings: In order to identify possible candidates among the EGFR ligands, we examined the predominant expression of an EGFR ligand in the chorionic villi and amniotic fluid during normal pregnancy, and analyzed the relationship between EGFR ligands and hCG in trophoblastic model cells. Amphiregulin was primarily expressed throughout human pregnancy and stimulated the secretion of hCG, indicating that amphiregulin is a key molecule among EGFR ligands. Significance: Amphiregulin may play a pivotal role in the development or maturation of placenta. Crown
AB - Aims: The aim of this study was to investigate the significance of epidermal growth factor receptor (EGFR) ligands produced in syncytiotrophoblasts during normal pregnancy. Main methods: We examined the expression of EGFR ligands in human pregnancy by real-time PCR, and analyzed the relationship between EGFR ligands and human chorionic gonadotropin (hCG) or human placental lactogen in amniotic fluid by ELISA. In addition, we also examined the EGFR ligands in syncytiotrophoblasts and the amount of hCG secretion in JAR, JEG3 and BeWo cells in the presence of each EGFR ligand. Key findings: In order to identify possible candidates among the EGFR ligands, we examined the predominant expression of an EGFR ligand in the chorionic villi and amniotic fluid during normal pregnancy, and analyzed the relationship between EGFR ligands and hCG in trophoblastic model cells. Amphiregulin was primarily expressed throughout human pregnancy and stimulated the secretion of hCG, indicating that amphiregulin is a key molecule among EGFR ligands. Significance: Amphiregulin may play a pivotal role in the development or maturation of placenta. Crown
UR - http://www.scopus.com/inward/record.url?scp=67349164260&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=67349164260&partnerID=8YFLogxK
U2 - 10.1016/j.lfs.2009.03.009
DO - 10.1016/j.lfs.2009.03.009
M3 - Article
C2 - 19344731
AN - SCOPUS:67349164260
SN - 0024-3205
VL - 84
SP - 796
EP - 804
JO - Life Sciences
JF - Life Sciences
IS - 23-24
ER -