Amounts of proteins altered by mutations in the dnaA gene of Escherichia coli

Akiko Ohba, Tohru Mizushima, Tsutomu Katayama, Kazuhisa Sekimizu

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


We identified proteins whose amounts were altered in a temperature-sensitive dnaA46 mutant of Escherichia coli. Proteins whose amounts were increased in the mutant were serine hydroxymethyltransferase, β-ketoacyl [acyl carrier protein] synthase II, long-chain fatty acid transport protein, and UDP-glucose 4-epimerase, while the decreased ones were flagellin and D-ribose-binding protein. Transformation of the mutant with a plasmid containing the wild type dnaA gene complemented the phenotype. As pulse-labeling experiments revealed that the rates of synthesis of the proteins were altered in the mutant, DnaA protein may be involved in expression of these proteins.

Original languageEnglish
Pages (from-to)125-128
Number of pages4
JournalFEBS Letters
Issue number2-3
Publication statusPublished - Mar 10 1997

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology


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