TY - JOUR
T1 - Amino acid sequence of pheromone-inducible surface protein in Enterococcus faecalis, that is encoded on the conjugative plasmid pPD1
AU - Nakayama, Jiro
AU - Nagasawa, Hiromich
AU - Isogai, Akira
AU - Clewell, Don B.
AU - Suzuki, Akinori
N1 - Funding Information:
AcknowledgementWs: e thank Dr. Ohtsubof or helpful discussions and Drs Chieko Kitada and MasahikoF ujino for providingt he synthetic peptidesc PD1. This work was partly supportedb y the grants from Ministry of Education,S ciencea nd Culture,J apan, by the Fu-jisawaF oundationT okyo, Japan and by public HealthS erviceG rant GM33956f rom the National Instituteso f Health, USA.
PY - 1990/7/30
Y1 - 1990/7/30
N2 - The major pheromone-inducible protein, PD78, believed to contribute to bacterial conjugation, was purified from Enterococcus (formerly Streptococcus) faecalis cells containing the plasmid pPD1. A cloned EcoRI-Bg1II 3.6-kbp fragment of the plasmid pAM351(pPd1::Tn916) contained an open reading frame corresponding to 467 amino acid residues representing PD78. In a central region of the deduced protein, there is a repeated sequence of X-X-Pro that is repeated 15 times. This is analogous to the Gin-Gin-Pro repeat in the C-tenninal region of TraD product encoded on the R 100 plasmid in Escherichia coli.
AB - The major pheromone-inducible protein, PD78, believed to contribute to bacterial conjugation, was purified from Enterococcus (formerly Streptococcus) faecalis cells containing the plasmid pPD1. A cloned EcoRI-Bg1II 3.6-kbp fragment of the plasmid pAM351(pPd1::Tn916) contained an open reading frame corresponding to 467 amino acid residues representing PD78. In a central region of the deduced protein, there is a repeated sequence of X-X-Pro that is repeated 15 times. This is analogous to the Gin-Gin-Pro repeat in the C-tenninal region of TraD product encoded on the R 100 plasmid in Escherichia coli.
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U2 - 10.1016/0014-5793(90)81019-K
DO - 10.1016/0014-5793(90)81019-K
M3 - Article
C2 - 2116976
AN - SCOPUS:0025302551
SN - 0014-5793
VL - 268
SP - 245
EP - 248
JO - FEBS Letters
JF - FEBS Letters
IS - 1
ER -