Amino acid and bioamine separations

Liquid chromatographic methods for the determination of amino acids and bioamines are outlined and discussed. In Section 4.2, direct separation methods for amino acids with postcolumn derivatization or MS/MS detection are described. In Section 4.3, typical indirect methods for amino acids are summarized. In this section, precolumn derivatization reagents for UV–Vis detection, fluorescence detection, and MS/MS detection are explained. Enantioselective analytical methods for amino acids are also described in Section 4.4. Direct and indirect separation methods for bioamines are summarized in Sections 4.5 and 4.6.