TY - JOUR
T1 - Ameloblastoma induces osteoclastogenesis
T2 - A possible role of ameloblastoma in expanding in the bone
AU - Sandra, Ferry
AU - Hendarmin, Laifa
AU - Kukita, Toshio
AU - Nakao, Yu
AU - Nakamura, Norifumi
AU - Nakamura, Seiji
N1 - Funding Information:
We thank Dr. Hidemitsu Harada for providing the AM-1 cells and Dr. Quanyong Tang for technical supports. This project was supported by Grant-in-Aid for Scientific Research from JSPS (No. P 03145).
PY - 2005/7
Y1 - 2005/7
N2 - Ameloblastoma, a tumor located in bone, when neglected, can perforate the bone and, ultimately, spread into the soft tissues. To expand in the bone, ameloblastoma must have a mechanism of resorbing the surrounding bone. However, the mechanism for bone resorption is poorly understood. In the present study, we found that RANKL and TNFα were expressed and secreted by ameloblastoma cells, and was proven to induce osteoclastogenesis. Our present results also showed that phosphorylation of p38, SAPK, p44/42 and Akt were upregulated under treatment of 10×CM (concentrated conditioned media of AM-1 cells). We also noticed formation of resorption lacunae on dentin slice by 10×CM-induced osteoclast-like MNCs. These results suggested that ameloblastoma by secreting RANKL and TNFα could induce osteoclastogenesis.
AB - Ameloblastoma, a tumor located in bone, when neglected, can perforate the bone and, ultimately, spread into the soft tissues. To expand in the bone, ameloblastoma must have a mechanism of resorbing the surrounding bone. However, the mechanism for bone resorption is poorly understood. In the present study, we found that RANKL and TNFα were expressed and secreted by ameloblastoma cells, and was proven to induce osteoclastogenesis. Our present results also showed that phosphorylation of p38, SAPK, p44/42 and Akt were upregulated under treatment of 10×CM (concentrated conditioned media of AM-1 cells). We also noticed formation of resorption lacunae on dentin slice by 10×CM-induced osteoclast-like MNCs. These results suggested that ameloblastoma by secreting RANKL and TNFα could induce osteoclastogenesis.
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U2 - 10.1016/j.oraloncology.2005.02.008
DO - 10.1016/j.oraloncology.2005.02.008
M3 - Article
C2 - 15935726
AN - SCOPUS:20444454718
SN - 1368-8375
VL - 41
SP - 637
EP - 644
JO - Oral Oncology
JF - Oral Oncology
IS - 6
ER -
