TY - JOUR
T1 - Agrobacterium-mediated transformation of japonica rice using mature embryos and regenerated transgenic plants
AU - Elakhdar, Ammar
AU - Fukuda, Masako
AU - Kubo, Takahiko
N1 - Funding Information:
This work was supported in part by a Grant-in-Aid for Scientific Research (KAKENHI) from the Japan Society for the Promotion of Science (Grant number: 19P19394). We are grateful to professor Calvin O. Qualset, University of California Davis, for reading the manuscript and providing comments and suggestions. This protocol is derived from previous publications (Satoh-Cruz et al., 2010; Fukuda et al., 2011 and 2013; Elakhdar et al., 2019).
Publisher Copyright:
© 2021 The Authors.
PY - 2021/9/20
Y1 - 2021/9/20
N2 - Identification of novel genes and their functions in rice is a critical step to improve economic traits. Agrobacterium tumefaciens-mediated transformation is a proven method in many laboratories and widely adopted for genetic engineering in rice. However, the efficiency of gene transfer by Agrobacterium in rice is low, particularly among japonica and indica varieties. In this protocol, we elucidate a rapid and highly efficient protocol to transform and regenerate transgenic rice plants through important key features of Agrobacterium transformation and standard regeneration media, especially enhancing culture conditions, timing, and growth hormones. With this protocol, transformed plantlets from the embryogenetic callus of the japonica cultivar 'Taichung 65' may be obtained within 90 days. This protocol may be used with other japonica rice varieties.
AB - Identification of novel genes and their functions in rice is a critical step to improve economic traits. Agrobacterium tumefaciens-mediated transformation is a proven method in many laboratories and widely adopted for genetic engineering in rice. However, the efficiency of gene transfer by Agrobacterium in rice is low, particularly among japonica and indica varieties. In this protocol, we elucidate a rapid and highly efficient protocol to transform and regenerate transgenic rice plants through important key features of Agrobacterium transformation and standard regeneration media, especially enhancing culture conditions, timing, and growth hormones. With this protocol, transformed plantlets from the embryogenetic callus of the japonica cultivar 'Taichung 65' may be obtained within 90 days. This protocol may be used with other japonica rice varieties.
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U2 - 10.21769/BioProtoc.4143
DO - 10.21769/BioProtoc.4143
M3 - Article
AN - SCOPUS:85116203807
SN - 2331-8325
VL - 11
JO - Bio-protocol
JF - Bio-protocol
IS - 18
M1 - e4143
ER -