TY - JOUR
T1 - Age-related spatial and nonspatial short-term memory in Cav2.1α1 mutant mice, Rolling Nagoya
AU - Takahashi, Eiki
AU - Niimi, Kimie
AU - Itakura, Chitoshi
N1 - Funding Information:
The Rolling Nagoya strain was provided by the RIKEN BRC with the support of the National BioResource Project of the Ministry of Education, Culture, Sports, Science, and Technology in Japan. Male rol/+ and +/+ F1 progeny were derived from a cross between rol/+ mice and genotyped by PCR using tail DNA [18] . The mice were given free access to water and food pellets (CRF-1, Oriental Yeast Co., Ltd., Tokyo, Japan) and were kept under a 12/12 h light/dark cycle (lights on from 08:00 to 20:00) at 23 ± 1 °C and 55 ± 5% humidity. All animal procedures were approved by the Animal Experiments Committee of RIKEN and were treated in accordance with the Institutional Guidelines for Experiments using Animals. We used 2-month-old (young) and 22-month-old (aged) rol/+ and +/+ mice for the object recognition test (young rol/+, +/+: n = 10, 10; aged rol/+, +/+: n = 10, 10), for an object location test (young rol/+, +/+: n = 10, 10; aged rol/+, +/+: n = 10, 10), and for a real-time qRT-PCR assay (young rol/+, +/+: n = 12, 12; aged rol/+, +/+: n = 12, 12). We used separate groups of male mice for each of the behavioral tests and the expression assay.
PY - 2009/12/1
Y1 - 2009/12/1
N2 - Aged heterozygous Rolling Nagoya mice carrying Cav2.1α1 mutation show deficits with regard to spatial short-term memory using hippocampus-related object location test, but not with regard to nonspatial memory using perirhinal cortex-related object recognition test. In hippocampus, wild-type Cav2.1α1 mRNA exhibited lower expression and mutant-type expression was higher in aged heterozygous mice. In perirhinal cortex, there were no significantly different expressions. Alteration of age-dependent expressions of Cav2.1 channels differs in different regions with related effects on behavioral performances.
AB - Aged heterozygous Rolling Nagoya mice carrying Cav2.1α1 mutation show deficits with regard to spatial short-term memory using hippocampus-related object location test, but not with regard to nonspatial memory using perirhinal cortex-related object recognition test. In hippocampus, wild-type Cav2.1α1 mRNA exhibited lower expression and mutant-type expression was higher in aged heterozygous mice. In perirhinal cortex, there were no significantly different expressions. Alteration of age-dependent expressions of Cav2.1 channels differs in different regions with related effects on behavioral performances.
UR - http://www.scopus.com/inward/record.url?scp=67849099110&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=67849099110&partnerID=8YFLogxK
U2 - 10.1016/j.bbr.2009.05.020
DO - 10.1016/j.bbr.2009.05.020
M3 - Article
C2 - 19467269
AN - SCOPUS:67849099110
SN - 0166-4328
VL - 204
SP - 241
EP - 245
JO - Behavioural Brain Research
JF - Behavioural Brain Research
IS - 1
ER -