TY - JOUR
T1 - Affinity purification of multifunctional oligomeric ligands synthesized
T2 - Via controlled radical polymerization
AU - Hoshino, Yu
AU - Shimohara, Shinnosuke
AU - Wada, Yusuke
AU - Nakamoto, Masahiko
AU - Miura, Yoshiko
N1 - Funding Information:
This research was supported by JSPS (23750193), JST A-STEP (AS2321466 and AS231Z01490D), AMED (JP17efk0108127h), JSPS KAKENHI (20H02789), Ogasawara Foundation, Kakihara Foundation, and Kao Foundation for Arts and Sciences.
Publisher Copyright:
© The Royal Society of Chemistry.
PY - 2020/7/14
Y1 - 2020/7/14
N2 - Abiotic oligomeric ligands with a strong affinity for a target peptide sequence were isolated by affinity purification from a pool of 30-mer acrylic random ter-oligomers that were synthesized via a controlled radical polymerization process. Our results indicate that the oligomeric ligands with suitable sequence and/or stereochemical configurations for the target can be isolated from as-polymerized random co-polymers based on the affinity to the target. This process will be a powerful tool for the development of stable and inexpensive ligands that can be used to detect, neutralize and purify proteins with a target epitope sequence.
AB - Abiotic oligomeric ligands with a strong affinity for a target peptide sequence were isolated by affinity purification from a pool of 30-mer acrylic random ter-oligomers that were synthesized via a controlled radical polymerization process. Our results indicate that the oligomeric ligands with suitable sequence and/or stereochemical configurations for the target can be isolated from as-polymerized random co-polymers based on the affinity to the target. This process will be a powerful tool for the development of stable and inexpensive ligands that can be used to detect, neutralize and purify proteins with a target epitope sequence.
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U2 - 10.1039/d0tb00849d
DO - 10.1039/d0tb00849d
M3 - Article
C2 - 32519727
AN - SCOPUS:85088045487
SN - 2050-750X
VL - 8
SP - 5597
EP - 5601
JO - Journal of Materials Chemistry B
JF - Journal of Materials Chemistry B
IS - 26
ER -