Adsorption of inflammatory cytokines using a heparin-coated extracorporeal circuit

Masanori Fujita, Masayuki Ishihara, Katsuaki Ono, Hidemi Hattori, Akira Kurita, Masafumi Shimizu, Atsuhiro Mitsumaru, Daisuke Segawa, Kazuhiro Hinokiyama, Yoshimasa Kusama, Makoto Kikuchi, Tadaaki Maehara

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17 Citations (Scopus)


Cardiopulmonary bypass (CPB) surgeries cause an increase in plasma inflammatory cytokines, such as tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) along with whole-body inflammatory responses. The inflammatory responses during a CPB treatment are reduced when using a heparin-coated extracorporeal circuit. Because many cytokines, growth factors, and complements are known to interact with heparin, the reduction of inflammatory responses by a heparin-coated circuit is likely to depend on this heparin-binding nature of the inflammatory cytokines. In this study, the inflammatory cytokines, TNF-α and IL-6, in fetal bovine serum (FBS) bound to a heparin-agarose beads (heparin beads)-column and the adsorptions were competitively inhibited on addition of heparin in a concentration-dependent manner. TNF-α in FBS required a higher concentration of heparin (50% concentration inhibition [IC50] > 20μg/ml) to inhibit adsorption to the heparin beads-column compared with IL-6, probably because of a stronger interaction between TNF-α and heparin-beads. TNF-α and IL-6 concentrations in human heparinized blood significantly increased after a CPB treatment. Although the adsorbed amount of IL-6 onto the heparin-coated circuit was low (less than 6% of free circulating IL-6), a significant amount of TNF-α adsorbed onto the circuit (23.9-755% of free circulating TNF-α). Therefore, the adsorption of inflammatory cytokines, especially TNF-α, onto the inner heparin-coated surface of an extracorporeal circuit may partly account for a reduction in inflammatory responses.

Original languageEnglish
Pages (from-to)1020-1025
Number of pages6
JournalArtificial Organs
Issue number12
Publication statusPublished - Dec 2002
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering


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