ADRP stimulates lipid accumulation and lipid droplet formation in murine fibroblasts

Minako Imamura, Toyoshi Inoguchi, Shoichiro Ikuyama, Susumu Taniguchi, Kunihisa Kobayashi, Naoki Nakashima, Hajime Nawata

Research output: Contribution to journalArticlepeer-review

240 Citations (Scopus)


Adipose differentiation-related protein (ADRP) is a lipid droplet-associated protein that is expressed early during adipose differentiation. The present study was undertaken to reveal the role of ADRP in adipose differentiation. In murine fibroblasts infected with green fluorescent protein (GFP)-ADRP fusion protein expression adenovirus vector, confocal microscopic analysis showed the number and size of lipid droplets apparently increased comparing with those of control cells. Overexpressed GFP-ADRP were mainly located at the surface of lipid droplets and appeared to be "ring-shaped." Triacylglycerol content was also significantly (P < 0.001) increased in GFP-ADRP-over-expressed cells compared with control cells. ADRP-induced lipid accumulation did not depend on adipocyte-specific gene induction, such as peroxisome proliferator-activated receptor-γ, lipoprotein lipase, or other lipogenic genes, including acyl-CoA synthetase, fatty acid-binding protein, and fatty acid transporter. In conclusion, ADRP stimulated lipid accumulation and lipid droplet formation without induction of other adipocyte-specific genes or other lipogenic genes in murine fibroblasts. The detailed molecular mechanisms of ADRP on lipid accumulation remain to be elucidated.

Original languageEnglish
Pages (from-to)E775-E783
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Issue number4 46-4
Publication statusPublished - Oct 2002

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)


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