TY - JOUR
T1 - Adiponectin inhibits induction of TNF-α/RANKL-stimulated NFATc1 via the AMPK signaling
AU - Yamaguchi, Noboru
AU - Kukita, Toshio
AU - Li, Yin Ji
AU - Kamio, Noriaki
AU - Fukumoto, Satoshi
AU - Nonaka, Kazuaki
AU - Ninomiya, Yuzo
AU - Hanazawa, Shigemasa
AU - Yamashita, Yoshihisa
N1 - Funding Information:
We thank Drs. I. Shimomura and N. Maeda for generously providing mouse globular adiponectin cDNA, and Drs. K. Sanematsu and Y. Murata for their technical expertise. This study was supported by Grant-in-Aid for Scientific Research (C) 17592184 from the Ministry of Education, Science, Sports, and Culture of Japan.
PY - 2008/2/6
Y1 - 2008/2/6
N2 - We investigated here whether adiponectin can exhibit an inhibitory effect on tumor necrosis factor-alpha (TNF-α)- and receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclastogenesis by using RAW264 cell D clone with a high efficiency to form osteoclasts. Globular adiponectin (gAd) strongly inhibited TNF-α/RANKL-induced differentiation of osteoclasts by interfering with TNF receptor-associated factor 6 production and calcium signaling; consequently, the induction of nuclear factor of activated T cells c1 (NFATc1) was strongly inhibited. Moreover, we observed that inhibition of AMP-activated protein kinase abrogated gAd inhibition for TNF-α/RANKL-induced NFATc1 expression. Our data suggest that adiponectin acts as a potent regulator of bone resorption observed in diseases associated with cytokine activation.
AB - We investigated here whether adiponectin can exhibit an inhibitory effect on tumor necrosis factor-alpha (TNF-α)- and receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclastogenesis by using RAW264 cell D clone with a high efficiency to form osteoclasts. Globular adiponectin (gAd) strongly inhibited TNF-α/RANKL-induced differentiation of osteoclasts by interfering with TNF receptor-associated factor 6 production and calcium signaling; consequently, the induction of nuclear factor of activated T cells c1 (NFATc1) was strongly inhibited. Moreover, we observed that inhibition of AMP-activated protein kinase abrogated gAd inhibition for TNF-α/RANKL-induced NFATc1 expression. Our data suggest that adiponectin acts as a potent regulator of bone resorption observed in diseases associated with cytokine activation.
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U2 - 10.1016/j.febslet.2007.12.037
DO - 10.1016/j.febslet.2007.12.037
M3 - Article
C2 - 18201570
AN - SCOPUS:38649123699
SN - 0014-5793
VL - 582
SP - 451
EP - 456
JO - FEBS Letters
JF - FEBS Letters
IS - 3
ER -