Adiponectin inhibits induction of TNF-α/RANKL-stimulated NFATc1 via the AMPK signaling

Noboru Yamaguchi, Toshio Kukita, Yin Ji Li, Noriaki Kamio, Satoshi Fukumoto, Kazuaki Nonaka, Yuzo Ninomiya, Shigemasa Hanazawa, Yoshihisa Yamashita

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81 Citations (Scopus)


We investigated here whether adiponectin can exhibit an inhibitory effect on tumor necrosis factor-alpha (TNF-α)- and receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclastogenesis by using RAW264 cell D clone with a high efficiency to form osteoclasts. Globular adiponectin (gAd) strongly inhibited TNF-α/RANKL-induced differentiation of osteoclasts by interfering with TNF receptor-associated factor 6 production and calcium signaling; consequently, the induction of nuclear factor of activated T cells c1 (NFATc1) was strongly inhibited. Moreover, we observed that inhibition of AMP-activated protein kinase abrogated gAd inhibition for TNF-α/RANKL-induced NFATc1 expression. Our data suggest that adiponectin acts as a potent regulator of bone resorption observed in diseases associated with cytokine activation.

Original languageEnglish
Pages (from-to)451-456
Number of pages6
JournalFEBS Letters
Issue number3
Publication statusPublished - Feb 6 2008

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology


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