TY - JOUR
T1 - Active role of small non-coding RNAs derived from SINE/B1 retrotransposon during early mouse development
AU - Ohnishi, Yusuke
AU - Totoki, Yasushi
AU - Toyoda, Atsushi
AU - Watanabe, Toshiaki
AU - Yamamoto, Yasuhiro
AU - Tokunaga, Katsushi
AU - Sakaki, Yoshiyuki
AU - Sasaki, Hiroyuki
AU - Hohjoh, Hirohiko
N1 - Funding Information:
Acknowledgments We would like to thank all the technical staff of the Sequence Technology Team at RIKEN Genomic Sciences Center (GSC) for their assistance. This work was supported by research grants from the Ministry of Health, Labour and Welfare of Japan, by Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science and by NIG Cooperative Research Program (2008-A60).
PY - 2012/2
Y1 - 2012/2
N2 - Small RNAs derived from repetitive sequences appear to play essential roles in mammalian gametogenesis and early development. In this study we focused on the short interspersed nucleotide element B1 (SINE/B1) small RNAs, which were zygotically expressed in pre-implantation mouse embryos; and we investigated whether the SINE/B1 small RNAs played an active role in gene silencing during early mouse development. The results indicated that the level of silencing activity involving the SINE/B1 small RNAs as mediators was significantly reduced in Dicer-knockdown mouse embryos. In addition, when the SINE/B1 small RNAs were mapped to a full-length SINE/B1 sequence, phase-distribution of the small RNAs appeared, suggesting possible enzymatic involvement. Therefore, our present study suggested that the zygotically expressed SINE/B1 small RNAs in pre-implantation mouse embryos contain active small RNAs, which were presumably processed by Dicer and involved in gene silencing.
AB - Small RNAs derived from repetitive sequences appear to play essential roles in mammalian gametogenesis and early development. In this study we focused on the short interspersed nucleotide element B1 (SINE/B1) small RNAs, which were zygotically expressed in pre-implantation mouse embryos; and we investigated whether the SINE/B1 small RNAs played an active role in gene silencing during early mouse development. The results indicated that the level of silencing activity involving the SINE/B1 small RNAs as mediators was significantly reduced in Dicer-knockdown mouse embryos. In addition, when the SINE/B1 small RNAs were mapped to a full-length SINE/B1 sequence, phase-distribution of the small RNAs appeared, suggesting possible enzymatic involvement. Therefore, our present study suggested that the zygotically expressed SINE/B1 small RNAs in pre-implantation mouse embryos contain active small RNAs, which were presumably processed by Dicer and involved in gene silencing.
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U2 - 10.1007/s11033-011-0815-1
DO - 10.1007/s11033-011-0815-1
M3 - Article
C2 - 21567196
AN - SCOPUS:84860327228
SN - 0301-4851
VL - 39
SP - 903
EP - 909
JO - Molecular biology reports
JF - Molecular biology reports
IS - 2
ER -