TY - JOUR
T1 - Activation of inward current associated with M-potassium current inhibition in m1-muscarinic receptor-transformed NG108-15 cells by KST-5452, a novel cognition enhancer
AU - Kishida, Hideyuki
AU - Yamamoto, Kozo
AU - Fuse, Yoshihide
AU - Noda, Mami
AU - Higashida, Haruhiro
PY - 1994/5/19
Y1 - 1994/5/19
N2 - The electrophysiological effects of KST-5452 [3-(m-phenoxybenzylidene)-quinuclidine], an M1 muscarinic acetylcholine receptor (muscarinic AChR) binding compound, were studied in NG108-15 neuroblastoma × glioma hybrid cells transfected with ml muscarinic AChR cDNA. Application of KST-5452 to m1-transformed NGPM1-27 cells elicited a sustained inward current associated with decreased conductance and reduced M-current relaxations at a holding potential of -20 mV. The KST-5452-induced responses were blocked by pirenzepine, suggesting that KST-5452 acts as a potent excitant via M1 muscarinic AChRs in brain neurons.
AB - The electrophysiological effects of KST-5452 [3-(m-phenoxybenzylidene)-quinuclidine], an M1 muscarinic acetylcholine receptor (muscarinic AChR) binding compound, were studied in NG108-15 neuroblastoma × glioma hybrid cells transfected with ml muscarinic AChR cDNA. Application of KST-5452 to m1-transformed NGPM1-27 cells elicited a sustained inward current associated with decreased conductance and reduced M-current relaxations at a holding potential of -20 mV. The KST-5452-induced responses were blocked by pirenzepine, suggesting that KST-5452 acts as a potent excitant via M1 muscarinic AChRs in brain neurons.
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U2 - 10.1016/0304-3940(94)90676-9
DO - 10.1016/0304-3940(94)90676-9
M3 - Article
C2 - 8084514
AN - SCOPUS:0028237499
SN - 0304-3940
VL - 172
SP - 119
EP - 121
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 1-2
ER -