Activation of human CD8+ suppressor T cells by an antigen-specific CD4+ T-cell line in vitro

Mitsuru Fukunaga, Kenji Hirayama, Takehiko Sasazuki

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


We generated an antigen (streptococcal cell wall antigen)-specific T-cell line from peripheral blood mononuclear cells of a healthy donor with an intermediate response to streptococcal cell wall antigen. Proliferation of the T-cell line was completely blocked by a monoclonal antibody to HLA-DR. This line activated autologous CD8+ T cells in an antigen-specific manner in the presence of autologous monocytes. This activation was mediated by a factor derived from this line and was blocked by a monoclonal antibody against HLA class I molecules. The resultant CD8+ T blasts showed antigen-nonspecific suppression but no cytolytic activity. This antigen-specific generation of the CD8+ T-cell line in vitro by the antigen-specific CD4+ T-cell line is expected to contribute to analyses of functions of CD8+ T-cell subsets, particularly in the down-regulating system, at both cellular and molecular levels.

Original languageEnglish
Pages (from-to)157-168
Number of pages12
JournalHuman Immunology
Issue number3
Publication statusPublished - Jul 1989
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology


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