Acetyl-CoA production by encapsulated pyruvate ferredoxin oxidoreductase in alginate hydrogels

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    10 Citations (Scopus)


    Pyruvate ferredoxin oxidoreductase from Citrobacter sp. S-77 (PFORS77) was purified in order to develop a method for acetyl-CoA production. Although the purified PFORS77showed high O2-sensitivity, the activity could be remarkably stabilized in anaerobic conditions. PFORS77was effectively immobilized on ceramic hydroxyapatite (PFORS77-HA) with an efficiency of more than 96%, however, after encapsulation of PFORS77-HA in alginate, the rate of catalytic acetyl-CoA production was highly reduced to 36% when compared to that of the free enzyme. However, the operational stability of the PFORS77-HA in alginate hydrogels was remarkable, retaining over 68% initial activity even after ten repeated cycles. The results suggested that the PFORS77-HA hydrogels have a high potential for biotechnological application.

    Original languageEnglish
    Pages (from-to)279-285
    Number of pages7
    JournalBioresource Technology
    Publication statusPublished - 2017

    All Science Journal Classification (ASJC) codes

    • Bioengineering
    • Environmental Engineering
    • Renewable Energy, Sustainability and the Environment
    • Waste Management and Disposal


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