Accentuation by pertussis toxin of the 5-hydroxytryptamine-induced potentiation of ATP-evoked responses in rat pheochromocytoma cells

Schuichi Koizumi; Makoto Ikeda; Ken Nakazawa; Kaori Inoue; Kunisuke Nagamatsu; Akira Hasegawa; Kazuhide Inoue

doi:10.1016/0304-3940(94)11125-3

Accentuation by pertussis toxin of the 5-hydroxytryptamine-induced potentiation of ATP-evoked responses in rat pheochromocytoma cells

Schuichi Koizumi, Makoto Ikeda, Ken Nakazawa, Kaori Inoue, Kunisuke Nagamatsu, Akira Hasegawa, Kazuhide Inoue

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

We previously demonstrated that 5-hydroxytryptamine (5-HT) enhances the cationic current activated by extracellular ATP in rat pheochromocytoma PC12 cells. We report here that pertussis toxin (PTX) modulates this 5-HT-dependent enhancement in these cells. 5-HT potentiated ATP-evoked intracellular Ca²⁺ concentration ([Ca]_i) rise and dopamine release over a concentration range from 1 to 100 μM. When cells were pre-treated with PTX, this potentiation was accentuated. Pretreatment with PTX also accentuated the 5-HT dependent enhancement of the ATP-activated current. These results suggest that the enhancement by 5-HT of the ATP-evoked responses is negatively regulated by a mechanism mediated through PTX-sensitive GTP-binding protein.

Original language	English
Pages (from-to)	104-107
Number of pages	4
Journal	Neuroscience Letters
Volume	183
Issue number	1-2
DOIs	https://doi.org/10.1016/0304-3940(94)11125-3
Publication status	Published - Jan 2 1995
Externally published	Yes

All Science Journal Classification (ASJC) codes

Neuroscience(all)

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10.1016/0304-3940(94)11125-3

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title = "Accentuation by pertussis toxin of the 5-hydroxytryptamine-induced potentiation of ATP-evoked responses in rat pheochromocytoma cells",

abstract = "We previously demonstrated that 5-hydroxytryptamine (5-HT) enhances the cationic current activated by extracellular ATP in rat pheochromocytoma PC12 cells. We report here that pertussis toxin (PTX) modulates this 5-HT-dependent enhancement in these cells. 5-HT potentiated ATP-evoked intracellular Ca2+ concentration ([Ca]i) rise and dopamine release over a concentration range from 1 to 100 μM. When cells were pre-treated with PTX, this potentiation was accentuated. Pretreatment with PTX also accentuated the 5-HT dependent enhancement of the ATP-activated current. These results suggest that the enhancement by 5-HT of the ATP-evoked responses is negatively regulated by a mechanism mediated through PTX-sensitive GTP-binding protein.",

author = "Schuichi Koizumi and Makoto Ikeda and Ken Nakazawa and Kaori Inoue and Kunisuke Nagamatsu and Akira Hasegawa and Kazuhide Inoue",

year = "1995",

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T1 - Accentuation by pertussis toxin of the 5-hydroxytryptamine-induced potentiation of ATP-evoked responses in rat pheochromocytoma cells

AU - Koizumi, Schuichi

AU - Ikeda, Makoto

AU - Nakazawa, Ken

AU - Inoue, Kaori

AU - Nagamatsu, Kunisuke

AU - Hasegawa, Akira

AU - Inoue, Kazuhide

PY - 1995/1/2

Y1 - 1995/1/2

N2 - We previously demonstrated that 5-hydroxytryptamine (5-HT) enhances the cationic current activated by extracellular ATP in rat pheochromocytoma PC12 cells. We report here that pertussis toxin (PTX) modulates this 5-HT-dependent enhancement in these cells. 5-HT potentiated ATP-evoked intracellular Ca2+ concentration ([Ca]i) rise and dopamine release over a concentration range from 1 to 100 μM. When cells were pre-treated with PTX, this potentiation was accentuated. Pretreatment with PTX also accentuated the 5-HT dependent enhancement of the ATP-activated current. These results suggest that the enhancement by 5-HT of the ATP-evoked responses is negatively regulated by a mechanism mediated through PTX-sensitive GTP-binding protein.

AB - We previously demonstrated that 5-hydroxytryptamine (5-HT) enhances the cationic current activated by extracellular ATP in rat pheochromocytoma PC12 cells. We report here that pertussis toxin (PTX) modulates this 5-HT-dependent enhancement in these cells. 5-HT potentiated ATP-evoked intracellular Ca2+ concentration ([Ca]i) rise and dopamine release over a concentration range from 1 to 100 μM. When cells were pre-treated with PTX, this potentiation was accentuated. Pretreatment with PTX also accentuated the 5-HT dependent enhancement of the ATP-activated current. These results suggest that the enhancement by 5-HT of the ATP-evoked responses is negatively regulated by a mechanism mediated through PTX-sensitive GTP-binding protein.

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Accentuation by pertussis toxin of the 5-hydroxytryptamine-induced potentiation of ATP-evoked responses in rat pheochromocytoma cells

Abstract

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