AC susceptibility of magnetic markers in suspension for liquid phase immunoassay

Keiji Enpuku; Tsuyoshi Tanaka; Yuya Tamai; Masaaki Matsuo

doi:10.1016/j.jmmm.2009.02.099

AC susceptibility of magnetic markers in suspension for liquid phase immunoassay

Keiji Enpuku, Tsuyoshi Tanaka, Yuya Tamai, Masaaki Matsuo

Superconductive Systems Engineering Laboratory

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

AC susceptibility of magnetic markers in solution was studied for biosensor applications. First, frequency dependence of the susceptibility was measured, and size distribution of the markers was estimated by analyzing the experimental result with the so-called singular value decomposition (SVD) method. The size distribution estimated with the magnetic measurement agreed with that obtained from conventional optical measurement. Next, susceptibility measurement was applied to the liquid-phase immunoassay without bound/free (B/F) separation. We performed the detection of biotin-coated polymer beads in suspension using avidin-coated magnetic markers. Changes of the susceptibility and the size distribution caused by the binding reaction were shown.

Original language	English
Pages (from-to)	1621-1624
Number of pages	4
Journal	Journal of Magnetism and Magnetic Materials
Volume	321
Issue number	10
DOIs	https://doi.org/10.1016/j.jmmm.2009.02.099
Publication status	Published - May 2009

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Condensed Matter Physics

Access to Document

10.1016/j.jmmm.2009.02.099

Cite this

@article{62e098e1370f46ba8e9bccdf60096c33,

title = "AC susceptibility of magnetic markers in suspension for liquid phase immunoassay",

abstract = "AC susceptibility of magnetic markers in solution was studied for biosensor applications. First, frequency dependence of the susceptibility was measured, and size distribution of the markers was estimated by analyzing the experimental result with the so-called singular value decomposition (SVD) method. The size distribution estimated with the magnetic measurement agreed with that obtained from conventional optical measurement. Next, susceptibility measurement was applied to the liquid-phase immunoassay without bound/free (B/F) separation. We performed the detection of biotin-coated polymer beads in suspension using avidin-coated magnetic markers. Changes of the susceptibility and the size distribution caused by the binding reaction were shown.",

author = "Keiji Enpuku and Tsuyoshi Tanaka and Yuya Tamai and Masaaki Matsuo",

year = "2009",

month = may,

doi = "10.1016/j.jmmm.2009.02.099",

language = "English",

volume = "321",

pages = "1621--1624",

journal = "Journal of Magnetism and Magnetic Materials",

issn = "0304-8853",

publisher = "Elsevier",

number = "10",

}

TY - JOUR

T1 - AC susceptibility of magnetic markers in suspension for liquid phase immunoassay

AU - Enpuku, Keiji

AU - Tanaka, Tsuyoshi

AU - Tamai, Yuya

AU - Matsuo, Masaaki

PY - 2009/5

Y1 - 2009/5

N2 - AC susceptibility of magnetic markers in solution was studied for biosensor applications. First, frequency dependence of the susceptibility was measured, and size distribution of the markers was estimated by analyzing the experimental result with the so-called singular value decomposition (SVD) method. The size distribution estimated with the magnetic measurement agreed with that obtained from conventional optical measurement. Next, susceptibility measurement was applied to the liquid-phase immunoassay without bound/free (B/F) separation. We performed the detection of biotin-coated polymer beads in suspension using avidin-coated magnetic markers. Changes of the susceptibility and the size distribution caused by the binding reaction were shown.

AB - AC susceptibility of magnetic markers in solution was studied for biosensor applications. First, frequency dependence of the susceptibility was measured, and size distribution of the markers was estimated by analyzing the experimental result with the so-called singular value decomposition (SVD) method. The size distribution estimated with the magnetic measurement agreed with that obtained from conventional optical measurement. Next, susceptibility measurement was applied to the liquid-phase immunoassay without bound/free (B/F) separation. We performed the detection of biotin-coated polymer beads in suspension using avidin-coated magnetic markers. Changes of the susceptibility and the size distribution caused by the binding reaction were shown.

UR - http://www.scopus.com/inward/record.url?scp=64249124441&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=64249124441&partnerID=8YFLogxK

U2 - 10.1016/j.jmmm.2009.02.099

DO - 10.1016/j.jmmm.2009.02.099

M3 - Article

AN - SCOPUS:64249124441

SN - 0304-8853

VL - 321

SP - 1621

EP - 1624

JO - Journal of Magnetism and Magnetic Materials

JF - Journal of Magnetism and Magnetic Materials

IS - 10

ER -

AC susceptibility of magnetic markers in suspension for liquid phase immunoassay

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this