A ti-plasmid-convertible λ phage vector system harboring the hygromycin B phosphotransferase gene

Takuichi Fuse; Hiroaki Kodama; Nobuaki Hayashida; Kazuo Shinozaki; Koh Iba

doi:10.5511/plantbiotechnology.15.227

A ti-plasmid-convertible λ phage vector system harboring the hygromycin B phosphotransferase gene

Takuichi Fuse, Hiroaki Kodama, Nobuaki Hayashida, Kazuo Shinozaki, Koh Iba

Informational Biology

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Abstract

We constructed new λ TI phage vectors, λTI3, λTI4, λTI5 and λTI6, which were automatically convertible into Ti-plasmid binary vectors by using the Cre- lox site-specific recombination system of bacteriophage PI. Hygromycin B phosphotransferase (HPT) gene was inserted in the T-DNA region of these ATI vectors as a plant selectable maker. Tobacco plants, which were transformed by Agrobacterium containing the Ti-plasmids resulted from the automatic conversion of the λTI vectors, could be tightly selected by hygromycin B. This λTI system will certainly provide a powerful tool for genetic complementation test of candidate genes obtained by positional cloning.

Original language	English
Pages (from-to)	227-230
Number of pages	4
Journal	Plant Biotechnology
Volume	15
Issue number	4
DOIs	https://doi.org/10.5511/plantbiotechnology.15.227
Publication status	Published - 1998

All Science Journal Classification (ASJC) codes

Biotechnology
Agronomy and Crop Science
Plant Science

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abstract = "We constructed new λ TI phage vectors, λTI3, λTI4, λTI5 and λTI6, which were automatically convertible into Ti-plasmid binary vectors by using the Cre- lox site-specific recombination system of bacteriophage PI. Hygromycin B phosphotransferase (HPT) gene was inserted in the T-DNA region of these ATI vectors as a plant selectable maker. Tobacco plants, which were transformed by Agrobacterium containing the Ti-plasmids resulted from the automatic conversion of the λTI vectors, could be tightly selected by hygromycin B. This λTI system will certainly provide a powerful tool for genetic complementation test of candidate genes obtained by positional cloning.",

author = "Takuichi Fuse and Hiroaki Kodama and Nobuaki Hayashida and Kazuo Shinozaki and Koh Iba",

year = "1998",

doi = "10.5511/plantbiotechnology.15.227",

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AU - Fuse, Takuichi

AU - Kodama, Hiroaki

AU - Hayashida, Nobuaki

AU - Shinozaki, Kazuo

AU - Iba, Koh

PY - 1998

Y1 - 1998

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AB - We constructed new λ TI phage vectors, λTI3, λTI4, λTI5 and λTI6, which were automatically convertible into Ti-plasmid binary vectors by using the Cre- lox site-specific recombination system of bacteriophage PI. Hygromycin B phosphotransferase (HPT) gene was inserted in the T-DNA region of these ATI vectors as a plant selectable maker. Tobacco plants, which were transformed by Agrobacterium containing the Ti-plasmids resulted from the automatic conversion of the λTI vectors, could be tightly selected by hygromycin B. This λTI system will certainly provide a powerful tool for genetic complementation test of candidate genes obtained by positional cloning.

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A ti-plasmid-convertible λ phage vector system harboring the hygromycin B phosphotransferase gene

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