A ternary conjugation system for the construction of DNA libraries for geobacillus kaustophilus HTA426

Hirokazu Suzuki; Keisuke Wada; Megumi Furukawa; Katsumi Doi; Toshihisa Ohshima

doi:10.1271/bbb.130492

A ternary conjugation system for the construction of DNA libraries for geobacillus kaustophilus HTA426

Hirokazu Suzuki
, Keisuke Wada
, Megumi Furukawa
, Katsumi Doi
, Toshihisa Ohshima

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

This report describes efficient plasmid uptake by the thermophile Geobacillus kaustophilus HTA426 by means of a ternary conjugation system, which was used to construct thermophile DNA libraries for G. kaustophilus and to identify the genes for orotidine-5'-phosphate decarboxylase by in vivo functional screening. The results indicate that the conjugation system is useful in constructing G. kaustophilus libraries, which are practical in identifying thermophile genes.

Original language	English
Pages (from-to)	2316-2318
Number of pages	3
Journal	Bioscience, Biotechnology and Biochemistry
Volume	77
Issue number	11
DOIs	https://doi.org/10.1271/bbb.130492
Publication status	Published - 2013
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Medicine

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10.1271/bbb.130492

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abstract = "This report describes efficient plasmid uptake by the thermophile Geobacillus kaustophilus HTA426 by means of a ternary conjugation system, which was used to construct thermophile DNA libraries for G. kaustophilus and to identify the genes for orotidine-5'-phosphate decarboxylase by in vivo functional screening. The results indicate that the conjugation system is useful in constructing G. kaustophilus libraries, which are practical in identifying thermophile genes.",

author = "Hirokazu Suzuki and Keisuke Wada and Megumi Furukawa and Katsumi Doi and Toshihisa Ohshima",

note = "Funding Information: This work was supported by JSPS KAKENHI Grant no. 25450105, and by the Institute for Fermentation, Osaka, Japan.",

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T1 - A ternary conjugation system for the construction of DNA libraries for geobacillus kaustophilus HTA426

AU - Suzuki, Hirokazu

AU - Wada, Keisuke

AU - Furukawa, Megumi

AU - Doi, Katsumi

AU - Ohshima, Toshihisa

N1 - Funding Information: This work was supported by JSPS KAKENHI Grant no. 25450105, and by the Institute for Fermentation, Osaka, Japan.

PY - 2013

Y1 - 2013

N2 - This report describes efficient plasmid uptake by the thermophile Geobacillus kaustophilus HTA426 by means of a ternary conjugation system, which was used to construct thermophile DNA libraries for G. kaustophilus and to identify the genes for orotidine-5'-phosphate decarboxylase by in vivo functional screening. The results indicate that the conjugation system is useful in constructing G. kaustophilus libraries, which are practical in identifying thermophile genes.

AB - This report describes efficient plasmid uptake by the thermophile Geobacillus kaustophilus HTA426 by means of a ternary conjugation system, which was used to construct thermophile DNA libraries for G. kaustophilus and to identify the genes for orotidine-5'-phosphate decarboxylase by in vivo functional screening. The results indicate that the conjugation system is useful in constructing G. kaustophilus libraries, which are practical in identifying thermophile genes.

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JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 11

ER -

A ternary conjugation system for the construction of DNA libraries for geobacillus kaustophilus HTA426

Abstract

All Science Journal Classification (ASJC) codes

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