A study on the surface hydrophobicity of lipases

Yoshiaki Sugimura; Kimitoshi Fukunaga; Takahiro Matsuno; Katsumi Nakao; Masahiro Goto; Fumiyuki Nakashio

doi:10.1016/S1369-703X(99)00072-8

A study on the surface hydrophobicity of lipases

Yoshiaki Sugimura
, Kimitoshi Fukunaga
, Takahiro Matsuno
, Katsumi Nakao
, Masahiro Goto
, Fumiyuki Nakashio

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

Surface properties, including surface net and local hydrophobicities, of bovine serum albumin, γ-globulin, and six lipases of different origins were evaluated using the aqueous two-phase partitioning method. Each showed a specific and characteristic pattern of surface properties. Correlations between the protein surface hydrophobicities and the coverages of the proteins by lipid-coating with a synthetic detergent, dioleyl glucosyl L- glutamate, were discussed. The results indicated that the surface net hydrophobicity of each protein was indicative of the affinity of the protein for the coating detergent applied in lipid-coating. (C) 2000 Elsevier Science S.A.

Original language	English
Pages (from-to)	123-128
Number of pages	6
Journal	Biochemical Engineering Journal
Volume	5
Issue number	2
DOIs	https://doi.org/10.1016/S1369-703X(99)00072-8
Publication status	Published - Jun 2000

All Science Journal Classification (ASJC) codes

Bioengineering
Biotechnology
Environmental Engineering
Biomedical Engineering

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10.1016/S1369-703X(99)00072-8

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title = "A study on the surface hydrophobicity of lipases",

abstract = "Surface properties, including surface net and local hydrophobicities, of bovine serum albumin, γ-globulin, and six lipases of different origins were evaluated using the aqueous two-phase partitioning method. Each showed a specific and characteristic pattern of surface properties. Correlations between the protein surface hydrophobicities and the coverages of the proteins by lipid-coating with a synthetic detergent, dioleyl glucosyl L- glutamate, were discussed. The results indicated that the surface net hydrophobicity of each protein was indicative of the affinity of the protein for the coating detergent applied in lipid-coating. (C) 2000 Elsevier Science S.A.",

author = "Yoshiaki Sugimura and Kimitoshi Fukunaga and Takahiro Matsuno and Katsumi Nakao and Masahiro Goto and Fumiyuki Nakashio",

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T1 - A study on the surface hydrophobicity of lipases

AU - Sugimura, Yoshiaki

AU - Fukunaga, Kimitoshi

AU - Matsuno, Takahiro

AU - Nakao, Katsumi

AU - Goto, Masahiro

AU - Nakashio, Fumiyuki

PY - 2000/6

Y1 - 2000/6

N2 - Surface properties, including surface net and local hydrophobicities, of bovine serum albumin, γ-globulin, and six lipases of different origins were evaluated using the aqueous two-phase partitioning method. Each showed a specific and characteristic pattern of surface properties. Correlations between the protein surface hydrophobicities and the coverages of the proteins by lipid-coating with a synthetic detergent, dioleyl glucosyl L- glutamate, were discussed. The results indicated that the surface net hydrophobicity of each protein was indicative of the affinity of the protein for the coating detergent applied in lipid-coating. (C) 2000 Elsevier Science S.A.

AB - Surface properties, including surface net and local hydrophobicities, of bovine serum albumin, γ-globulin, and six lipases of different origins were evaluated using the aqueous two-phase partitioning method. Each showed a specific and characteristic pattern of surface properties. Correlations between the protein surface hydrophobicities and the coverages of the proteins by lipid-coating with a synthetic detergent, dioleyl glucosyl L- glutamate, were discussed. The results indicated that the surface net hydrophobicity of each protein was indicative of the affinity of the protein for the coating detergent applied in lipid-coating. (C) 2000 Elsevier Science S.A.

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M3 - Article

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SP - 123

EP - 128

JO - Biochemical Engineering Journal

JF - Biochemical Engineering Journal

IS - 2

ER -

A study on the surface hydrophobicity of lipases

Abstract

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