A Study of Cytochrome c Oxidase in Lipid Bilayer Membranes on Electrode Surfaces

John K. Cullison; Fred M. Hawkridge; Naotoshi Nakashima; Shimichi Yoshikawa

doi:10.1021/la00015a044

A Study of Cytochrome c Oxidase in Lipid Bilayer Membranes on Electrode Surfaces

John K. Cullison, Fred M. Hawkridge, Naotoshi Nakashima, Shimichi Yoshikawa

Research output: Contribution to journal › Article › peer-review

91 Citations (Scopus)

Abstract

A membrane-associated enzyme, cytochrome c oxidase, has been put in lipid bilayer membranes attached to gold electrode surfaces. Gold/thiol self-assembly chemistry and deoxycholate dialysis procedures have been used to insert cytochrome c oxidase into a lipid bilayer on gold surfaces with a controlled orientation. Voltammetric and spectroelectrochemical results indicate that direct electron-transfer communication between the gold electrode surface and cytochrome c oxidase has been achieved. Moreover, immobilized cytochrome c oxidase can both reduce and oxidize solution-resident cytochrome c by appropriately controlling the applied electrode potential.

Original language	English
Pages (from-to)	877-882
Number of pages	6
Journal	Langmuir
Volume	10
Issue number	3
DOIs	https://doi.org/10.1021/la00015a044
Publication status	Published - Mar 1 1994

All Science Journal Classification (ASJC) codes

Materials Science(all)
Condensed Matter Physics
Surfaces and Interfaces
Spectroscopy
Electrochemistry

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10.1021/la00015a044

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AU - Hawkridge, Fred M.

AU - Nakashima, Naotoshi

AU - Yoshikawa, Shimichi

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Y1 - 1994/3/1

N2 - A membrane-associated enzyme, cytochrome c oxidase, has been put in lipid bilayer membranes attached to gold electrode surfaces. Gold/thiol self-assembly chemistry and deoxycholate dialysis procedures have been used to insert cytochrome c oxidase into a lipid bilayer on gold surfaces with a controlled orientation. Voltammetric and spectroelectrochemical results indicate that direct electron-transfer communication between the gold electrode surface and cytochrome c oxidase has been achieved. Moreover, immobilized cytochrome c oxidase can both reduce and oxidize solution-resident cytochrome c by appropriately controlling the applied electrode potential.

AB - A membrane-associated enzyme, cytochrome c oxidase, has been put in lipid bilayer membranes attached to gold electrode surfaces. Gold/thiol self-assembly chemistry and deoxycholate dialysis procedures have been used to insert cytochrome c oxidase into a lipid bilayer on gold surfaces with a controlled orientation. Voltammetric and spectroelectrochemical results indicate that direct electron-transfer communication between the gold electrode surface and cytochrome c oxidase has been achieved. Moreover, immobilized cytochrome c oxidase can both reduce and oxidize solution-resident cytochrome c by appropriately controlling the applied electrode potential.

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JO - Langmuir

JF - Langmuir

IS - 3

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A Study of Cytochrome c Oxidase in Lipid Bilayer Membranes on Electrode Surfaces

Abstract

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