TY - GEN
T1 - A small-sized lipid nanoparticles production method using microfluidic devices with baffle structures
AU - Kimura, Niko
AU - Maeki, Masatoshi
AU - Note, Yusuke
AU - Sato, Yusuke
AU - Ishida, Akihiko
AU - Tani, Hirofumi
AU - Harashima, Hideyoshi
AU - Tokeshi, Manabu
N1 - Funding Information:
A part of this work was supported by Tokyo Kasei Chemical Promotion Foundation.
Publisher Copyright:
© 17CBMS-0001.
PY - 2020
Y1 - 2020
N2 - This paper reports a lipid nanoparticles (LNP) production method and its formation behavior using microfluidic devices with baffle structures. The microfluidic devices showed great mixing efficiency at 500 μL/min, and we achieved 20 nm-sized LNPs production that chaotic micromixers were not able to produce at the same flow rate condition. Additionally, we found that the smaller-sized LNPs/siRNA prepared by baffle structures have higher penetration efficiency than that of the larger-sized LNPs, but all of them showed the gene silencing activity. The microfluidic devices with baffle structures are expected to be a practicable apparatus for DDSs application.
AB - This paper reports a lipid nanoparticles (LNP) production method and its formation behavior using microfluidic devices with baffle structures. The microfluidic devices showed great mixing efficiency at 500 μL/min, and we achieved 20 nm-sized LNPs production that chaotic micromixers were not able to produce at the same flow rate condition. Additionally, we found that the smaller-sized LNPs/siRNA prepared by baffle structures have higher penetration efficiency than that of the larger-sized LNPs, but all of them showed the gene silencing activity. The microfluidic devices with baffle structures are expected to be a practicable apparatus for DDSs application.
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M3 - Conference contribution
AN - SCOPUS:85079686548
T3 - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
SP - 965
EP - 966
BT - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
PB - Chemical and Biological Microsystems Society
T2 - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
Y2 - 22 October 2017 through 26 October 2017
ER -