A single fluorophore-labeled aptamer sensor for the detection of interferon gamma

Siti Norulhuda Hashim; Akira Tsuchiya; Noriho Kamiya; Shinsuke Sando

doi:10.1246/cl.150794

A single fluorophore-labeled aptamer sensor for the detection of interferon gamma

Siti Norulhuda Hashim, Akira Tsuchiya, Noriho Kamiya, Shinsuke Sando

Applied Chemistry

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Interferon gamma (IFN-γ) is a cytokine that is mainly secreted from immune cells. It plays an important role in regulating neighboring cellular activities through interactions with cell membrane proteins. Because of its biological importance, there has been increasing demand for a biosensor that can respond to IFN-γ. Here, we report a fluorescent aptamer sensor that targets IFN-γ. The 25-nt IFN-γ aptamer was converted into a turn-on fluorescent sensor by labeling the 3'-end with fluorescein. The designed sensor achieved fluorescence sensing of IFN-γ with nanomolar affinity.

Original language	English
Pages (from-to)	1670-1672
Number of pages	3
Journal	Chemistry Letters
Volume	44
Issue number	12
DOIs	https://doi.org/10.1246/cl.150794
Publication status	Published - 2015

All Science Journal Classification (ASJC) codes

Chemistry(all)

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10.1246/cl.150794

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title = "A single fluorophore-labeled aptamer sensor for the detection of interferon gamma",

abstract = "Interferon gamma (IFN-γ) is a cytokine that is mainly secreted from immune cells. It plays an important role in regulating neighboring cellular activities through interactions with cell membrane proteins. Because of its biological importance, there has been increasing demand for a biosensor that can respond to IFN-γ. Here, we report a fluorescent aptamer sensor that targets IFN-γ. The 25-nt IFN-γ aptamer was converted into a turn-on fluorescent sensor by labeling the 3'-end with fluorescein. The designed sensor achieved fluorescence sensing of IFN-γ with nanomolar affinity.",

author = "Hashim, {Siti Norulhuda} and Akira Tsuchiya and Noriho Kamiya and Shinsuke Sando",

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AU - Hashim, Siti Norulhuda

AU - Tsuchiya, Akira

AU - Kamiya, Noriho

AU - Sando, Shinsuke

PY - 2015

Y1 - 2015

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AB - Interferon gamma (IFN-γ) is a cytokine that is mainly secreted from immune cells. It plays an important role in regulating neighboring cellular activities through interactions with cell membrane proteins. Because of its biological importance, there has been increasing demand for a biosensor that can respond to IFN-γ. Here, we report a fluorescent aptamer sensor that targets IFN-γ. The 25-nt IFN-γ aptamer was converted into a turn-on fluorescent sensor by labeling the 3'-end with fluorescein. The designed sensor achieved fluorescence sensing of IFN-γ with nanomolar affinity.

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A single fluorophore-labeled aptamer sensor for the detection of interferon gamma

Abstract

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