TY - GEN
T1 - A single cell nucleus array to monitor messenger-RNA molecule transportation through nuclear membrane
AU - Kaji, Noritada
AU - Koyama, Ryo
AU - Yasui, Takao
AU - Higashiyama, Tetsuya
AU - Baba, Yoshinobu
N1 - Funding Information:
This work was partly supported by the Nitto Foundation, the Nanotechnology Platform Program (Molecule and Material Synthesis) of the Ministry of Education, Culture, Sports, Science and Technology (MEXT), the JSPS Grant-in-Aid for Scientific Research (A) 24241050, and the JST under the ERATO Higashiyama Live-Holonics Project.
Publisher Copyright:
© 15CBMS-0001.
PY - 2015
Y1 - 2015
N2 - In this study, a microchamber array device was designed to capture a single cell, lyse a cell membrane, and isolate a single cell nucleus, and then mRNA in a single cell nucleus were directly quantitated by using highly sensitive In-Stem Molecular Beacons (ISMB). As a result, the expression of three frequently used house-keeping genes were successfully quantitated to an accuracy of 100 molecules within a single cell nucleus.
AB - In this study, a microchamber array device was designed to capture a single cell, lyse a cell membrane, and isolate a single cell nucleus, and then mRNA in a single cell nucleus were directly quantitated by using highly sensitive In-Stem Molecular Beacons (ISMB). As a result, the expression of three frequently used house-keeping genes were successfully quantitated to an accuracy of 100 molecules within a single cell nucleus.
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M3 - Conference contribution
AN - SCOPUS:84983356231
T3 - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 832
EP - 834
BT - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
Y2 - 25 October 2015 through 29 October 2015
ER -