A role for leukemia inhibitory factor in melanoma-induced bone metastasis

Shigeaki Maruta, Soichi Takiguchi, Miho Ueyama, Yasufumi Kataoka, Yoshinao Oda, Masazumi Tsuneyoshi, Haruo Iguchi

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)


Melanoma is commonly associated with multi-organ metastasis, and bone is a frequent metastatic site for melanoma. However, the mechanism responsible for such melanoma-induced bone metastasis is still poorly understood. In the present study, the intracardiac inoculation of leukemia inhibitory factor (LIF)-producing human melanoma-derived cells (SEKI) developed osteolytic bone destruction in male BALB/cA-nu/nu nude mice. To elucidate the role of LIF in melanoma-induced osteolysis, cells were prepared in which the expression of LIF was reduced using a siRNA technique from the parent SEKI cells. Osteoclastogenesis was induced in the co-culture of LIF and/or SEKI cells with osteoblastic stromal cells in vitro, whereas the LIF-reduced SEKI cells did not induce osteoclastogenesis. The intracardiac inoculation of LIF-reduced SEKI cells resulted in a significant reduction in the incidence and number of bone metastasis in comparison to those in the mice inoculated with the parent SEKI cells. The expression of LIF was found in seven of nine human melanoma-derived cell lines, suggesting that LIF expression is a universal event in melanoma. These findings suggest that a potential role for LIF in the melanoma-induced bone metastasis possibly through the stimulation of osteoclastogenesis. LIF might therefore be a potentially effective drug target in the treatment of bone metastasis in melanoma.

Original languageEnglish
Pages (from-to)133-141
Number of pages9
JournalClinical and Experimental Metastasis
Issue number2
Publication statusPublished - Feb 2009

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research


Dive into the research topics of 'A role for leukemia inhibitory factor in melanoma-induced bone metastasis'. Together they form a unique fingerprint.

Cite this