The integration of biosensing technology and soft robots can improve the diagnostic and therapeutic functions of bionic machines, and provide wearable devices with flexible, low-invasive, real-time monitoring biosensing functions. Here, a robotic electrochemical biosensor based on kinetic electronics technique was developed. The film robot is composed of a bimorph actuator layer and a biosensing function layer. The sensor function layer adopts sputtering technology to form a gold electrode, and is processed to form a Nafion-coated glucose oxidase electrode. The film robot could be used as a kinetic printed circuit for probe chips for biological and chemical experiments, and can realize bending action under an applied voltage of 5 V.