A RhoA and Rnd3 cycle regulates actin reassembly during membrane blebbing

Kana Aoki, Fumiyo Maeda, Tomoya Nagasako, Yuki Mochizuki, Seiichi Uchida, Junichi Ikenouchi

Research output: Contribution to journalArticlepeer-review

48 Citations (Scopus)


The actin cytoskeleton usually lies beneath the plasma membrane. When the membrane-associated actin cytoskeleton is transiently disrupted or the intracellular pressure is increased, the plasma membrane detaches from the cortex and protrudes. Such protruded membrane regions are called blebs. However, the molecular mechanisms underlying membrane blebbing are poorly understood. This study revealed that epidermal growth factor receptor kinase substrate 8 (Eps8) and ezrin are important regulators of rapid actin reassembly for the initiation and retraction of protruded blebs. Live-cell imaging of membrane blebbing revealed that local reassembly of actin filaments occurred at Eps8- and activated ezrin-positive foci of membrane blebs. Furthermore, we found that a RhoA-ROCK-Rnd3 feedback loop determined the local reassembly sites of the actin cortex during membrane blebbing.

Original languageEnglish
Pages (from-to)E1863-E1871
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number13
Publication statusPublished - Mar 29 2016

All Science Journal Classification (ASJC) codes

  • General


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