A rat monoclonal antibody against the chromatin remodeling factor CHD5

Saori Yoshimura; Tomohiko Yoshimi; Yasuyuki Ohkawa; Masayuki Azuma; Taro Tachibana

doi:10.1089/hyb.2009.0069

A rat monoclonal antibody against the chromatin remodeling factor CHD5

Saori Yoshimura, Tomohiko Yoshimi, Yasuyuki Ohkawa, Masayuki Azuma, Taro Tachibana

Medical Research Center Initiative for High Depth Omics

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

CHD5 (chromodomain/helicase/DNA-binding protein 5) is a member of the CHD subfamily of chromatin remodeling Swi/Snf proteins, and has been recently identified as a tumor suppressor in a diverse range of human cancers. We report here on the establishment of a hybridoma cell line for producing a monoclonal antibody against CHD5 by the rat medial iliac lymph node method. Immunoblotting analyses indicated that this antibody, MAb 5A10, specifically recognizes endogenous CHD5. In immunostaining using the antibody, a nuclear staining pattern was observed. The monoclonal antibody will be useful in immunoblotting and immunolocalization experiments in a variety of cells and tissues, as well as in further studies of the biological function and cellular dynamics of this protein.

Original language	English
Pages (from-to)	63-66
Number of pages	4
Journal	Hybridoma
Volume	29
Issue number	1
DOIs	https://doi.org/10.1089/hyb.2009.0069
Publication status	Published - Feb 1 2010

All Science Journal Classification (ASJC) codes

Immunology and Allergy
Immunology

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1089/hyb.2009.0069

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AU - Yoshimura, Saori

AU - Yoshimi, Tomohiko

AU - Ohkawa, Yasuyuki

AU - Azuma, Masayuki

AU - Tachibana, Taro

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AB - CHD5 (chromodomain/helicase/DNA-binding protein 5) is a member of the CHD subfamily of chromatin remodeling Swi/Snf proteins, and has been recently identified as a tumor suppressor in a diverse range of human cancers. We report here on the establishment of a hybridoma cell line for producing a monoclonal antibody against CHD5 by the rat medial iliac lymph node method. Immunoblotting analyses indicated that this antibody, MAb 5A10, specifically recognizes endogenous CHD5. In immunostaining using the antibody, a nuclear staining pattern was observed. The monoclonal antibody will be useful in immunoblotting and immunolocalization experiments in a variety of cells and tissues, as well as in further studies of the biological function and cellular dynamics of this protein.

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A rat monoclonal antibody against the chromatin remodeling factor CHD5

Abstract

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