A rare point mutation in the Ras oncogene in hepatocellular carcinoma

Akinobu Taketomi, Ken Shirabe, Jun Muto, Shohei Yoshiya, Takashi Motomura, Yohei Mano, Toru Ikegami, Tomoharu Yoshizumi, Kenji Sugio, Yoshihiko Maehara

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)


Purpose: The Ras gene is one of the oncogenes most frequently detected in human cancers, and codes for three proteins (K-, N-, and H-Ras). The aim of this study was to examine the mutations in codons 12, 13 and 61 of the three Ras genes in cases of human hepatocellular carcinoma (HCC). Methods: Paired samples of HCC and corresponding non-malignant liver tissue were collected from 61 patients who underwent hepatectomy. A dot-blot analysis was used to analyze the products of the polymerase chain reaction (PCR) amplification of codons 12, 13, and 61 of K-, N- and H-Ras for mutations. Results: Only one mutation (K-Ras codon 13; Gly to Asp) was detected among the 61 patients. Interestingly, this patient had a medical history of surgery for both gastric cancer and right lung cancer. No mutations were found in codons 12 and 61 of K-Ras or codons 12, 13 and 61 of the N-Ras and H-Ras genes in any of the HCCs or corresponding non-malignant tissues. Conclusions: These findings indicated that the activation of Ras proto-oncogenes by mutations in codons 12, 13, and 61 does not play a major role in hepatocellular carcinogenesis.

Original languageEnglish
Pages (from-to)289-292
Number of pages4
JournalSurgery today
Issue number3
Publication statusPublished - Mar 2013

All Science Journal Classification (ASJC) codes

  • Surgery


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