A rapid and quantitative detection of cellular protein kinase activity based on MALDI-TOF-MS

Yuki Otsubo; Hiromu Ikeda; Junpei Kamimoto; Takuro Niidome; Takeshi Mori; Yoshiki Katayama

doi:10.1246/cl.131227

A rapid and quantitative detection of cellular protein kinase activity based on MALDI-TOF-MS

Yuki Otsubo, Hiromu Ikeda, Junpei Kamimoto, Takuro Niidome, Takeshi Mori, Yoshiki Katayama

Research output: Contribution to journal › Article › peer-review

Abstract

A rapid and quantitative measurement of protein kinase activity based on MALDI-TOF-MS was developed. Here, biotinylated substrate peptides with stable isotope labeling were used to enable the rapid and specific enrichment of the peptides. Protein kinase activities in two samples can be directly compared utlizing the mass-unit difference of the subsrate peptides with different isotope labels. We successfully evaluated the effect of an inhibitory drug on the cellular protein kinase acitivity.

Original language	English
Pages (from-to)	658-660
Number of pages	3
Journal	Chemistry Letters
Volume	43
Issue number	5
DOIs	https://doi.org/10.1246/cl.131227
Publication status	Published - 2014

All Science Journal Classification (ASJC) codes

Chemistry(all)

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abstract = "A rapid and quantitative measurement of protein kinase activity based on MALDI-TOF-MS was developed. Here, biotinylated substrate peptides with stable isotope labeling were used to enable the rapid and specific enrichment of the peptides. Protein kinase activities in two samples can be directly compared utlizing the mass-unit difference of the subsrate peptides with different isotope labels. We successfully evaluated the effect of an inhibitory drug on the cellular protein kinase acitivity.",

author = "Yuki Otsubo and Hiromu Ikeda and Junpei Kamimoto and Takuro Niidome and Takeshi Mori and Yoshiki Katayama",

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AU - Otsubo, Yuki

AU - Ikeda, Hiromu

AU - Kamimoto, Junpei

AU - Niidome, Takuro

AU - Mori, Takeshi

AU - Katayama, Yoshiki

PY - 2014

Y1 - 2014

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A rapid and quantitative detection of cellular protein kinase activity based on MALDI-TOF-MS

Abstract

All Science Journal Classification (ASJC) codes

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