TY - JOUR
T1 - A pulsed-field gel electrophoresis typing scheme for Vibrio parahaemolyticus isolates from fifteen countries
AU - Wong, Hin chung
AU - Liu, Shu Hui
AU - Chiou, Chien Shun
AU - Nishibuchi, Mitsuaki
AU - Lee, Bok Kwon
AU - Suthienkul, Orasa
AU - Nair, Gopinath Balakrish
AU - Kaysner, Charles A.
AU - Taniguchi, Hatsumi
N1 - Funding Information:
The authors would like to thank the Department of Health of the Republic of China for financially supporting this research under Contract No. DOH89-TD-1053 and DOH90-TD-1075.
PY - 2007/3/20
Y1 - 2007/3/20
N2 - Vibrio parahaemolyticus is an important foodborne pathogen in Taiwan and many other maritime Asian countries where seafood is frequently consumed. A total of 535 strains of V. parahaemolyticus were recovered mostly (97%) from clinical samples obtained in Taiwan or in 14 other countries. These strains were typed by pulsed-field gel electrophoresis following SfiI digestion and a typing scheme was generated. The 115 different patterns identified were grouped into 13 types with dissimilarity values less than 15, plus 16 miscellaneous patterns not grouped into any of the types. Types I, A, D and J contained the most patterns, with the numbers of patterns being 17, 13, 12, and 11, respectively. However, types I, B, D, A, H and C contained the most strains, with the numbers of strains being 204, 73, 71, 54, 29 and 25, respectively. Type I consisted exclusively of the pandemic O3:K6 strains and genetically closely related strains. This PFGE typing scheme for V. parahaemolyticus could be used for the characterization of pathogenic isolates.
AB - Vibrio parahaemolyticus is an important foodborne pathogen in Taiwan and many other maritime Asian countries where seafood is frequently consumed. A total of 535 strains of V. parahaemolyticus were recovered mostly (97%) from clinical samples obtained in Taiwan or in 14 other countries. These strains were typed by pulsed-field gel electrophoresis following SfiI digestion and a typing scheme was generated. The 115 different patterns identified were grouped into 13 types with dissimilarity values less than 15, plus 16 miscellaneous patterns not grouped into any of the types. Types I, A, D and J contained the most patterns, with the numbers of patterns being 17, 13, 12, and 11, respectively. However, types I, B, D, A, H and C contained the most strains, with the numbers of strains being 204, 73, 71, 54, 29 and 25, respectively. Type I consisted exclusively of the pandemic O3:K6 strains and genetically closely related strains. This PFGE typing scheme for V. parahaemolyticus could be used for the characterization of pathogenic isolates.
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U2 - 10.1016/j.ijfoodmicro.2006.09.024
DO - 10.1016/j.ijfoodmicro.2006.09.024
M3 - Article
C2 - 17161487
AN - SCOPUS:33847194636
SN - 0168-1605
VL - 114
SP - 280
EP - 287
JO - International Journal of Food Microbiology
JF - International Journal of Food Microbiology
IS - 3
ER -