TY - JOUR
T1 - A possible maternal-effect mutant of Xenopus laevis
T2 - II. Studies of RNA synthesis in dissociated embryonic cells
AU - Shiokawa, Koichiro
AU - Tashiro, Kosuke
AU - Nakakura, Norihiko
AU - Fu, Yuchang
AU - Atsuchi, Yasuo
AU - Nakazato, Sakiko
AU - Tsuzaki, Yoshinari
AU - Ikenishi, Kohji
N1 - Funding Information:
We thank Professor K. Yamana for his warm encouragement during the present study. The present work was supported, in part, by a Grant-in-Aid for Scientific Research (No. 61540523)t o KS. from the Ministry of Education, Science and Culture of Japan and a grant from Takeda Science Foundation (1986) to K.S.
PY - 1988/9
Y1 - 1988/9
N2 - Embryos from a female of Xenopus laevis (designated as no. 65) arrest development at gastrulation and are assumed to be ova-deficient mutant. We dissociated these embryos and studied RNA synthesis at different stages. The cells from the ova-deficient embryos reaggregated quite actively as wild-type embryo cells until the late gastrula stage. RNA synthesis was normal at the early blastula stage but greatly inhibited by the late blastula (stage 9.5) stage, when the synthesis of DNA and protein was still not inhibited appreciably. Thus, inhibition in RNA synthesis appears to be the first manifestation of the maternal defect that occurs before the gastrulation arrest.
AB - Embryos from a female of Xenopus laevis (designated as no. 65) arrest development at gastrulation and are assumed to be ova-deficient mutant. We dissociated these embryos and studied RNA synthesis at different stages. The cells from the ova-deficient embryos reaggregated quite actively as wild-type embryo cells until the late gastrula stage. RNA synthesis was normal at the early blastula stage but greatly inhibited by the late blastula (stage 9.5) stage, when the synthesis of DNA and protein was still not inhibited appreciably. Thus, inhibition in RNA synthesis appears to be the first manifestation of the maternal defect that occurs before the gastrulation arrest.
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U2 - 10.1016/0922-3371(88)90054-8
DO - 10.1016/0922-3371(88)90054-8
M3 - Article
C2 - 2461791
AN - SCOPUS:0023777706
SN - 0922-3371
VL - 25
SP - 47
EP - 55
JO - Cell Differentiation and Development
JF - Cell Differentiation and Development
IS - 1
ER -