A photoregulated ATP generation system for in vitro motility assay

Arif Md Rashedul Kabir; Masaki Ito; Kyohei Uenishi; Shizuka Anan; Akihiko Konagaya; Kazuki Sada; Miwa Sugiura; Akira Kakugo

doi:10.1246/cl.160903

A photoregulated ATP generation system for in vitro motility assay

Arif Md Rashedul Kabir, Masaki Ito, Kyohei Uenishi, Shizuka Anan, Akihiko Konagaya, Kazuki Sada, Miwa Sugiura, Akira Kakugo

Research output: Contribution to journal › Article › peer-review

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Abstract

We have demonstrated in vitro motility assay of microtubules on a kinesin-coated substrate by using adenosine triphosphate (ATP) generated from microsphere gels containing thylakoid membranes. Upon photoirradiation, the gels generated ATP, which was utilized for performing gliding motions of microtubules. This work may facilitate the development of a localized ATP generation system in the in vitro motility assay, which consequently would widen the applications of biomolecular motors in nanotechnology.

Original language	English
Pages (from-to)	178-180
Number of pages	3
Journal	Chemistry Letters
Volume	46
Issue number	2
DOIs	https://doi.org/10.1246/cl.160903
Publication status	Published - 2017
Externally published	Yes

All Science Journal Classification (ASJC) codes

Chemistry(all)

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10.1246/cl.160903

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title = "A photoregulated ATP generation system for in vitro motility assay",

abstract = "We have demonstrated in vitro motility assay of microtubules on a kinesin-coated substrate by using adenosine triphosphate (ATP) generated from microsphere gels containing thylakoid membranes. Upon photoirradiation, the gels generated ATP, which was utilized for performing gliding motions of microtubules. This work may facilitate the development of a localized ATP generation system in the in vitro motility assay, which consequently would widen the applications of biomolecular motors in nanotechnology.",

author = "Kabir, {Arif Md Rashedul} and Masaki Ito and Kyohei Uenishi and Shizuka Anan and Akihiko Konagaya and Kazuki Sada and Miwa Sugiura and Akira Kakugo",

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T1 - A photoregulated ATP generation system for in vitro motility assay

AU - Kabir, Arif Md Rashedul

AU - Ito, Masaki

AU - Uenishi, Kyohei

AU - Anan, Shizuka

AU - Konagaya, Akihiko

AU - Sada, Kazuki

AU - Sugiura, Miwa

AU - Kakugo, Akira

N1 - Funding Information: This work was financially supported by a Grant-in-Aid for Scientific Research on Innovative Areas (JSPS KAKENHI Grant Number JP24104004) from Japan Society for the Promotion of Science (JSPS). Publisher Copyright: © 2017 The Chemical Society of Japan.

PY - 2017

Y1 - 2017

N2 - We have demonstrated in vitro motility assay of microtubules on a kinesin-coated substrate by using adenosine triphosphate (ATP) generated from microsphere gels containing thylakoid membranes. Upon photoirradiation, the gels generated ATP, which was utilized for performing gliding motions of microtubules. This work may facilitate the development of a localized ATP generation system in the in vitro motility assay, which consequently would widen the applications of biomolecular motors in nanotechnology.

AB - We have demonstrated in vitro motility assay of microtubules on a kinesin-coated substrate by using adenosine triphosphate (ATP) generated from microsphere gels containing thylakoid membranes. Upon photoirradiation, the gels generated ATP, which was utilized for performing gliding motions of microtubules. This work may facilitate the development of a localized ATP generation system in the in vitro motility assay, which consequently would widen the applications of biomolecular motors in nanotechnology.

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DO - 10.1246/cl.160903

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SP - 178

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JO - Chemistry Letters

JF - Chemistry Letters

IS - 2

ER -

A photoregulated ATP generation system for in vitro motility assay

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