A palm-sized surface plasmon resonance sensor with microchip flow cell

Hizuru Nakajima, Yuuki Harada, Yasukazu Asano, Tatsuro Nakagama, Katsumi Uchiyama, Toshihiko Imato, Nobuaki Soh, Akihide Hemmi

    Research output: Contribution to journalArticlepeer-review

    22 Citations (Scopus)


    A small-sized surface plasmon resonance (SPR) sensor with a microchip flow cell has been developed for the purpose of enhancing the sensitivity of the SPR detector for low molecular weight compounds. This portable differential SPR detector consisted of an LED, two cylindrical lenses, a round prism, a divided mirror, a CCD, electronics, and a polydimethylsiloxane/gold microchip with two flow paths (10 mm long, 1 mm wide, 20-100 μm deep). 3-Mercaptopropyltrimethoxysilane was used for sealing the microchip. The performance of the on-site orientated SPR detector was estimated using sucrose and IgA. A drastic change in the SPR intensity appeared. The depth of the flow cell was in inverse proportion to the SPR intensity. Compared to a conventional flow cell having the size of 10 mm (L) × 1 mm (W) × 1 mm (D), its sensitivity to 10% sucrose and 0.9 nM IgA increased about 11 and 39 times, respectively. This phenomenon seemed to be due to the increase in the substance on the SPR sensor based on its size effect. These results showed that the application of the microchip sensor for SPR measurement has the possibility for improvement of the SPR intensity for low molecular substances.

    Original languageEnglish
    Pages (from-to)419-425
    Number of pages7
    Issue number2
    Publication statusPublished - Sept 15 2006

    All Science Journal Classification (ASJC) codes

    • Analytical Chemistry


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