A nuclear factor which interacts with an at-cluster in the first intron of rat α2-macroglobulin gene

Takashi Ito; Yoshiyuki Sakaki

doi:10.1016/0006-291X(87)91004-7

A nuclear factor which interacts with an at-cluster in the first intron of rat α₂-macroglobulin gene

Takashi Ito
, Yoshiyuki Sakaki

Bioregulation

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

We have identified a nuclear protein which binds specifically to the first intron of rat α₂-macroglobulin gene. The protein became insoluble at low salt concentration retaining the binding specificity. Its molecular weight was estimated to be 22 kilodalton by a protein blotting procedure. The binding site of the protein determined by DNase I footprinting was an AT-strech which shared 80% homology with the cleavage consensus of Drosophila topoisomerase II.

Original language	English
Pages (from-to)	824-830
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	147
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(87)91004-7
Publication status	Published - Sept 15 1987

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(87)91004-7

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title = "A nuclear factor which interacts with an at-cluster in the first intron of rat α2-macroglobulin gene",

abstract = "We have identified a nuclear protein which binds specifically to the first intron of rat α2-macroglobulin gene. The protein became insoluble at low salt concentration retaining the binding specificity. Its molecular weight was estimated to be 22 kilodalton by a protein blotting procedure. The binding site of the protein determined by DNase I footprinting was an AT-strech which shared 80\% homology with the cleavage consensus of Drosophila topoisomerase II.",

author = "Takashi Ito and Yoshiyuki Sakaki",

note = "Funding Information: providing We would the nucleotide like to thank sequence Drs. Y. data, Tsuchiya and Misses and M. H. Hattori Hamada and for Y. Hayashi for assistance in preparation of the manuscript. This work was supported part by the Grant-in-Aid for Cancer Research from the Ministry of Education, Culture and Sciences (Japan).",

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AU - Ito, Takashi

AU - Sakaki, Yoshiyuki

N1 - Funding Information: providing We would the nucleotide like to thank sequence Drs. Y. data, Tsuchiya and Misses and M. H. Hattori Hamada and for Y. Hayashi for assistance in preparation of the manuscript. This work was supported part by the Grant-in-Aid for Cancer Research from the Ministry of Education, Culture and Sciences (Japan).

PY - 1987/9/15

Y1 - 1987/9/15

N2 - We have identified a nuclear protein which binds specifically to the first intron of rat α2-macroglobulin gene. The protein became insoluble at low salt concentration retaining the binding specificity. Its molecular weight was estimated to be 22 kilodalton by a protein blotting procedure. The binding site of the protein determined by DNase I footprinting was an AT-strech which shared 80% homology with the cleavage consensus of Drosophila topoisomerase II.

AB - We have identified a nuclear protein which binds specifically to the first intron of rat α2-macroglobulin gene. The protein became insoluble at low salt concentration retaining the binding specificity. Its molecular weight was estimated to be 22 kilodalton by a protein blotting procedure. The binding site of the protein determined by DNase I footprinting was an AT-strech which shared 80% homology with the cleavage consensus of Drosophila topoisomerase II.

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ER -

A nuclear factor which interacts with an at-cluster in the first intron of rat α₂-macroglobulin gene

Abstract

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