A novel streptomyces restriction endonuclease, Sse1825I, cleaving at 5′-GG/GWCCC-3′

Yoshiko Nomura; Yoshizumi Ishino; Fusao Kimizuka; Ikunoshin Kato

doi:10.1016/0378-1119(95)00119-Q

A novel streptomyces restriction endonuclease, Sse1825I, cleaving at 5′-GG/GWCCC-3′

Yoshiko Nomura
, Yoshizumi Ishino
, Fusao Kimizuka
, Ikunoshin Kato

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

We isolated and characterized from a Streptomyces species a new class-II restriction endonuclease, which recognizes the palindromic heptanucleotide sequence 5′-G G↓G W C C C 3′-C C C W G↑G G (where W = A or T) and cleaves double-stranded DNA after the second G in this sequence This Sse18251 enzyme cleaves phage λ DNA at one site, adenovirus type 2 DNA at eight sites, but does not cleave pBR322, SV40, CoIE1, pUC18 and pUC19, and replicative forms of M13mpl8 and M13mpl9, and ΦX174 DNAs.

Original language	English
Pages (from-to)	323-324
Number of pages	2
Journal	Gene
Volume	157
Issue number	1-2
DOIs	https://doi.org/10.1016/0378-1119(95)00119-Q
Publication status	Published - May 19 1995
Externally published	Yes

All Science Journal Classification (ASJC) codes

Genetics

Access to Document

10.1016/0378-1119(95)00119-Q

Cite this

@article{b5200679e1834d8f8526d164f09da757,

title = "A novel streptomyces restriction endonuclease, Sse1825I, cleaving at 5′-GG/GWCCC-3′",

abstract = "We isolated and characterized from a Streptomyces species a new class-II restriction endonuclease, which recognizes the palindromic heptanucleotide sequence 5′-G G↓G W C C C 3′-C C C W G↑G G (where W = A or T) and cleaves double-stranded DNA after the second G in this sequence This Sse18251 enzyme cleaves phage λ DNA at one site, adenovirus type 2 DNA at eight sites, but does not cleave pBR322, SV40, CoIE1, pUC18 and pUC19, and replicative forms of M13mpl8 and M13mpl9, and ΦX174 DNAs.",

author = "Yoshiko Nomura and Yoshizumi Ishino and Fusao Kimizuka and Ikunoshin Kato",

year = "1995",

month = may,

day = "19",

doi = "10.1016/0378-1119(95)00119-Q",

language = "English",

volume = "157",

pages = "323--324",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier B.V.",

number = "1-2",

}

TY - JOUR

T1 - A novel streptomyces restriction endonuclease, Sse1825I, cleaving at 5′-GG/GWCCC-3′

AU - Nomura, Yoshiko

AU - Ishino, Yoshizumi

AU - Kimizuka, Fusao

AU - Kato, Ikunoshin

PY - 1995/5/19

Y1 - 1995/5/19

N2 - We isolated and characterized from a Streptomyces species a new class-II restriction endonuclease, which recognizes the palindromic heptanucleotide sequence 5′-G G↓G W C C C 3′-C C C W G↑G G (where W = A or T) and cleaves double-stranded DNA after the second G in this sequence This Sse18251 enzyme cleaves phage λ DNA at one site, adenovirus type 2 DNA at eight sites, but does not cleave pBR322, SV40, CoIE1, pUC18 and pUC19, and replicative forms of M13mpl8 and M13mpl9, and ΦX174 DNAs.

AB - We isolated and characterized from a Streptomyces species a new class-II restriction endonuclease, which recognizes the palindromic heptanucleotide sequence 5′-G G↓G W C C C 3′-C C C W G↑G G (where W = A or T) and cleaves double-stranded DNA after the second G in this sequence This Sse18251 enzyme cleaves phage λ DNA at one site, adenovirus type 2 DNA at eight sites, but does not cleave pBR322, SV40, CoIE1, pUC18 and pUC19, and replicative forms of M13mpl8 and M13mpl9, and ΦX174 DNAs.

UR - https://www.scopus.com/pages/publications/0029016158

UR - https://www.scopus.com/pages/publications/0029016158#tab=citedBy

U2 - 10.1016/0378-1119(95)00119-Q

DO - 10.1016/0378-1119(95)00119-Q

M3 - Article

C2 - 7607520

AN - SCOPUS:0029016158

SN - 0378-1119

VL - 157

SP - 323

EP - 324

JO - Gene

JF - Gene

IS - 1-2

ER -

A novel streptomyces restriction endonuclease, Sse1825I, cleaving at 5′-GG/GWCCC-3′

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this